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Magnetic iron oxide nanoparticles modified with vanadate and phosphate salts for purification of alkaline phosphatase from the bovine skim milk.
- Source :
-
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2019 Mar 01; Vol. 175, pp. 644-653. Date of Electronic Publication: 2018 Dec 19. - Publication Year :
- 2019
-
Abstract
- Modified Fe <subscript>3</subscript> O <subscript>4</subscript> magnetic nanoparticles (magnetic nanocarrier) technology have found the proper place in separation and purification techniques, such as protein and enzyme purification, mostly due to its easy and fast operational procedure by using an external permanent magnet. Herein, Fe <subscript>3</subscript> O <subscript>4</subscript> magnetic nanoparticles were prepared, and surface modification was performed with vanadate and phosphate salts to yield four various model of magnetic nanocarriers. Affinity ligands which are used for immobilization on the nanocarriers leading to the development of appropriative nanocarriers for the affinity separation of alkaline phosphatase from the bovine milk. The findings showed that the use of sodium hexametaphosphate affinity ligand attached to the carrier with an 18-atom linker leads to better separation of alkaline phosphatase from the bovine milk with 14.1-fold purification efficiency. All results confirmed that our designed nanocarriers can purify alkaline phosphatase using a fast and low-cost approach.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cattle
Chromatography, Affinity methods
Phosphates chemistry
Surface Properties
Vanadates chemistry
Alkaline Phosphatase isolation & purification
Ferrosoferric Oxide chemistry
Magnetite Nanoparticles chemistry
Milk chemistry
Milk Proteins isolation & purification
Solid Phase Extraction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4367
- Volume :
- 175
- Database :
- MEDLINE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 30590325
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2018.12.051