Back to Search
Start Over
Evaluation of the Chemopreventive Activity of Grape Skin Extract Using Medium-term Oral Carcinogenesis Assay Induced by 4-Nitroquinoline 1-Oxide.
- Source :
-
Anticancer research [Anticancer Res] 2019 Jan; Vol. 39 (1), pp. 177-182. - Publication Year :
- 2019
-
Abstract
- Background/aim: The aim of this study was to evaluate the chemoprotective potential of grape skin extract following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO).<br />Materials and Methods: Male Wistar rats were distributed into four groups (n=5, per group): Control Group: free access to commercial diet and drinking water for 12 weeks; 4NQO Group: received 4NQO diluted in drinking water daily, for 12 weeks; Grape Skin Extract Group: free access to water and received grape skin extract incorporated with diet for 12 weeks; 4NQO + Grape Skin Extract Group: received 4NQO in drinking water daily and grape extract incorporated with diet for 12 weeks.<br />Results: Animals treated with grape skin extract revealed a significant reduction in epithelial dysplasia. Also, 8-hydroxy-2'-deoxyguanosine (8-OHdG) and ki-67 immunoexpression was reduced in animals treated with grape skin extract. Western blot analysis showed a significant decrease of p-NFκB p50 and MyD88 protein expression in the groups treated with grape skin extract. Copper-zinc superoxide dismutase, manganese superoxide dismutase, and catalase gene expression did not present any statistically significant differences (p>0.05).<br />Conclusion: Grape skin extract displayed chemopreventive activity in oral carcinogenesis assays as depicted by its antioxidant, anti-proliferative and anti-inflammatory properties.<br /> (Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Subjects :
- 4-Nitroquinoline-1-oxide toxicity
Animals
Antioxidants administration & dosage
Antioxidants chemistry
Catalase genetics
Gene Expression Regulation, Neoplastic drug effects
Humans
Mouth Neoplasms chemically induced
Mouth Neoplasms pathology
Plant Extracts chemistry
Rats
Superoxide Dismutase genetics
Superoxide Dismutase-1 genetics
Carcinogenesis drug effects
Mouth Neoplasms drug therapy
Plant Extracts administration & dosage
Vitis chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 39
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 30591456
- Full Text :
- https://doi.org/10.21873/anticanres.13095