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A Phytophthora Effector Suppresses Trans-Kingdom RNAi to Promote Disease Susceptibility.
- Source :
-
Cell host & microbe [Cell Host Microbe] 2019 Jan 09; Vol. 25 (1), pp. 153-165.e5. Date of Electronic Publication: 2018 Dec 27. - Publication Year :
- 2019
-
Abstract
- RNA silencing (RNAi) has a well-established role in anti-viral immunity in plants. The destructive eukaryotic pathogen Phytophthora encodes suppressors of RNAi (PSRs), which enhance plant susceptibility. However, the role of small RNAs in defense against eukaryotic pathogens is unclear. Here, we show that Phytophthora infection of Arabidopsis leads to increased production of a diverse pool of secondary small interfering RNAs (siRNAs). Instead of regulating endogenous plant genes, these siRNAs are found in extracellular vesicles and likely silence target genes in Phytophthora during natural infection. Introduction of a plant siRNA in Phytophthora leads to developmental deficiency and abolishes virulence, while Arabidopsis mutants defective in secondary siRNA biogenesis are hypersusceptible. Notably, Phytophthora effector PSR2 specifically inhibits secondary siRNA biogenesis in Arabidopsis and promotes infection. These findings uncover the role of siRNAs as antimicrobial agents against eukaryotic pathogens and highlight a defense/counter-defense arms race centered on trans-kingdom gene silencing between hosts and pathogens.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Arabidopsis genetics
Arabidopsis microbiology
Arabidopsis Proteins genetics
Gene Expression Regulation, Plant
Gene Silencing
Genes, Reporter genetics
Host-Pathogen Interactions genetics
Host-Pathogen Interactions immunology
MicroRNAs genetics
MicroRNAs immunology
Phosphoprotein Phosphatases antagonists & inhibitors
Phosphoprotein Phosphatases metabolism
Plant Diseases microbiology
Plant Immunity genetics
Plant Immunity immunology
Plant Leaves immunology
Plant Leaves microbiology
RNA, Small Interfering biosynthesis
RNA, Small Interfering drug effects
RNA, Small Interfering genetics
RNA-Binding Proteins genetics
RNA-Binding Proteins metabolism
Nicotiana
Verticillium
Virulence
Virulence Factors genetics
Virulence Factors metabolism
Arabidopsis immunology
Disease Susceptibility microbiology
Phytophthora metabolism
Phytophthora pathogenicity
Plant Diseases immunology
RNA Interference immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1934-6069
- Volume :
- 25
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell host & microbe
- Publication Type :
- Academic Journal
- Accession number :
- 30595554
- Full Text :
- https://doi.org/10.1016/j.chom.2018.11.007