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Imaging the Dynamics of Mitophagy in Live Cells.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1880, pp. 601-610. - Publication Year :
- 2019
-
Abstract
- Investigating the precise spatiotemporal dynamics of mitophagy can provide insights into how mitochondrial quality control is regulated in different tissues and organisms. Here, we outline live imaging assays to quantitatively assess mitophagy dynamics in real time. This protocol describes both chemical and optogenetic techniques to induce mitochondrial damage with high spatial and temporal control. Using these assays, mitochondria can be tracked from before they sustain damage up to their engulfment by autophagosomes and acidification by lysosomes.
- Subjects :
- Cell Cycle Proteins
Fluorescent Dyes chemistry
Genes, Reporter genetics
HeLa Cells
Humans
Intravital Microscopy instrumentation
Luminescent Proteins chemistry
Luminescent Proteins genetics
Lysosomes metabolism
Membrane Transport Proteins
Microscopy, Confocal instrumentation
Microscopy, Confocal methods
Microtubule-Associated Proteins genetics
Microtubule-Associated Proteins metabolism
Optogenetics instrumentation
Spatio-Temporal Analysis
Time-Lapse Imaging instrumentation
Time-Lapse Imaging methods
Transcription Factor TFIIIA genetics
Transcription Factor TFIIIA metabolism
Ubiquitin-Protein Ligases genetics
Ubiquitin-Protein Ligases metabolism
Autophagosomes metabolism
Intravital Microscopy methods
Mitochondria metabolism
Mitophagy physiology
Optogenetics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1880
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 30610725
- Full Text :
- https://doi.org/10.1007/978-1-4939-8873-0_39