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Gene expression profiling identifies potential molecular markers of papillary thyroid carcinoma.
- Source :
-
Cancer biomarkers : section A of Disease markers [Cancer Biomark] 2019; Vol. 24 (1), pp. 71-83. - Publication Year :
- 2019
-
Abstract
- Background: Thyroid cancer is the most common endocrine malignancy worldwide, with the predominant form papillary thyroid carcinoma (PTC) representing approximately 80% of cases.<br />Objective: This study was addressed to identify potential genes and pathways involved in the pathogenesis of PTC and potential novel biomarkers for this disease.<br />Methods: Gene expression profiling was carried out by DNA microarray technology. Validation of microarray data by qRT-PCR, western blot, and enzyme linked immunosorbent assay was also performed in a selected set of genes and gene products, with the potential to be used as diagnostic or prognostic biomarkers, such as those associated with cell adhesion, extracellular matrix (ECM) remodeling and immune/inflammatory response.<br />Results: In this study we found that upregulation of extracellular activities, such as proteoglycans, ECM-receptor interaction, and cell adhesion molecules, were the most prominent feature of PTC. Significantly over-expressed genes included SDC1 (syndecan 1), SDC4 (syndecan 4), KLK7 (kallikrein-related peptidase 7), KLK10 (kallikrein-related peptidase 10), SLPI (secretory leukocyte peptidase inhibitor), GDF15 (growth/differentiation factor-15), ALOX5 (arachidonate 5-lipoxygenase), SFRP2 (secreted Frizzled-related protein 2), among others. Further, elevated KLK10 levels were detected in patients with PTC. Many of these genes belong to KEGG pathway "Proteoglycans in cancer".<br />Conclusions: Using DNA microarray analysis allowed the identification of genes and pathways with known important roles in malignant transformation, and also the discovery of novel genes that may be potential biomarkers for PTC.
- Subjects :
- Adult
Aged
Female
Humans
Male
Middle Aged
Neoplasm Staging
Thyroid Cancer, Papillary metabolism
Thyroid Cancer, Papillary pathology
Thyroid Cancer, Papillary therapy
Thyroid Neoplasms metabolism
Thyroid Neoplasms pathology
Thyroid Neoplasms therapy
Young Adult
Biomarkers, Tumor
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Thyroid Cancer, Papillary genetics
Thyroid Neoplasms genetics
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 1875-8592
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cancer biomarkers : section A of Disease markers
- Publication Type :
- Academic Journal
- Accession number :
- 30614796
- Full Text :
- https://doi.org/10.3233/CBM-181758