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SPP1 inhibition improves the cisplatin chemo-sensitivity of cervical cancer cell lines.
- Source :
-
Cancer chemotherapy and pharmacology [Cancer Chemother Pharmacol] 2019 Apr; Vol. 83 (4), pp. 603-613. Date of Electronic Publication: 2019 Jan 09. - Publication Year :
- 2019
-
Abstract
- Purpose: Cisplatin (DDP)-based chemotherapy is a standard strategy for cervical cancer, while chemoresistance remains a huge challenge. In the present study, we aimed to explore the effects of SPP1 on the proliferation and apoptosis rate of the HeLa cervical cancer cell line with cisplatin (DDP) resistance.<br />Methods: Microarray analysis was employed to select differentially expressed genes in cervical cancer tissues and adjacent tissues. Then, we established a DDP-resistant HeLa cell line (res-HeLa). Western blotting was used to detect SPP1 expression in both tissue and cells. After the transfection with si-SPP1 and pcDNA3.1-SPP1, colony formation and MTT assays were applied to detect cell proliferation changes. Flow cytometry was employed to detect the cell apoptosis rate. Western blotting was performed to verify the activation of PI3K/Akt signal pathway proteins related to DDP resistance.<br />Results: SPP1 was overexpressed in cervical cancer tissues and cell lines. Compared to normal HeLa cells, expression of SPP1 was significantly enhanced in res-HeLa cells. SPP1 knockdown resulted in repressed proliferation and enhanced apoptosis of res-HeLa cells, which could be reversed by SPP1 overexpression in HeLa cells. Additionally, downregulation of SPP1 improved the DDP sensitivity of HeLa by inhibiting the PI3K/Akt signaling pathway.<br />Conclusion: SPP1 inhibition could suppress proliferation, induce apoptosis and increase the DDP chemo-sensitivity of HeLa cells.
- Subjects :
- Cell Proliferation drug effects
Down-Regulation
Drug Resistance, Neoplasm
Female
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
HeLa Cells
Humans
Microarray Analysis
Osteopontin genetics
Uterine Cervical Neoplasms pathology
Antineoplastic Agents pharmacology
Apoptosis drug effects
Cisplatin pharmacology
Osteopontin antagonists & inhibitors
Uterine Cervical Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0843
- Volume :
- 83
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cancer chemotherapy and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 30627777
- Full Text :
- https://doi.org/10.1007/s00280-018-3759-5