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A dynamic and integrated epigenetic program at distal regions orchestrates transcriptional responses to VEGFA.
- Source :
-
Genome research [Genome Res] 2019 Feb; Vol. 29 (2), pp. 193-207. Date of Electronic Publication: 2019 Jan 22. - Publication Year :
- 2019
-
Abstract
- Cell behaviors are dictated by epigenetic and transcriptional programs. Little is known about how extracellular stimuli modulate these programs to reshape gene expression and control cell behavioral responses. Here, we interrogated the epigenetic and transcriptional response of endothelial cells to VEGFA treatment and found rapid chromatin changes that mediate broad transcriptomic alterations. VEGFA-responsive genes were associated with active promoters, but changes in promoter histone marks were not tightly linked to gene expression changes. VEGFA altered transcription factor occupancy and the distal epigenetic landscape, which profoundly contributed to VEGFA-dependent changes in gene expression. Integration of gene expression, dynamic enhancer, and transcription factor occupancy changes induced by VEGFA yielded a VEGFA-regulated transcriptional regulatory network, which revealed that the small MAF transcription factors are master regulators of the VEGFA transcriptional program and angiogenesis. Collectively these results revealed that extracellular stimuli rapidly reconfigure the chromatin landscape to coordinately regulate biological responses.<br /> (© 2019 Wang et al.; Published by Cold Spring Harbor Laboratory Press.)
- Subjects :
- Animals
Cells, Cultured
Chromatin metabolism
Enhancer Elements, Genetic
Humans
Maf Transcription Factors metabolism
Male
Mice
Mice, Nude
Promoter Regions, Genetic
Transcription Factors metabolism
Epigenesis, Genetic
Neovascularization, Physiologic genetics
Transcription, Genetic
Vascular Endothelial Growth Factor A physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1549-5469
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Genome research
- Publication Type :
- Academic Journal
- Accession number :
- 30670628
- Full Text :
- https://doi.org/10.1101/gr.239053.118