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Combined cellular and soluble mediator analysis for improved diagnosis of vitreoretinal lymphoma.
- Source :
-
Acta ophthalmologica [Acta Ophthalmol] 2019 Sep; Vol. 97 (6), pp. 626-632. Date of Electronic Publication: 2019 Jan 27. - Publication Year :
- 2019
-
Abstract
- Purpose: Primary vitreoretinal lymphoma [(P)VRL]) is a rare malignancy of the eye localized in the retina, vitreous or choroid. Here, we aim to determine the value of the combination of innovative diagnostic methods for accurate differentiation between (P)VRL and non-(P)VRL in patients with suspect uveitis or vitritis.<br />Methods: Multicolour flow cytometric immunophenotyping of cells in the vitreous samples was performed using the EuroFlow small sample tube. Additionally, cytokines/chemokines and growth factors were measured in the vitreous specimens using a multiplex immunoassay. Data were evaluated in predefined clinical subgroups using omniviz unsupervised Pearson's correlation visualization and unsupervised heatmap analysis.<br />Results: A total of 53 patients were prospectively included in the period 2012-2015. In the (P)VRL subgroup (n = 10), nine cases showed aberrant surface membrane immunoglobulin (SmIg) light chain expression. In the non-(P)VRL group (n = 43) clearly skewed SmIg light chain expression was observed in two multiple sclerosis-related uveitis cases, but not in other uveitis types. Soluble mediator measurement revealed high interleukin (IL)-10/IL-6 ratios, and high IL-1RA levels in 9/10 (P)VRL cases, but not in any non-(P)VRL case. Further correlation and heatmap analysis revealed a minimal signature of cellular parameters (CD19+ B cells, aberrant SmIg light chain expression) and cytokine parameters (IL-10/IL-6 ratio >1, high IL-10, high IL-1 RA, high monocyte chemotactic protein-1, high macrophage inflammatory protein-1β) to reliably distinguish (P)VRL from non-(P)VRL.<br />Conclusion: Here, we show the power of a combined cellular and proteomics strategy for detecting (P)VRL in vitreous specimens, especially in cases with minor cellular (P)VRL infiltrates.<br /> (© 2019 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.)
- Subjects :
- Biomarkers, Tumor metabolism
Choroid metabolism
Choroid Neoplasms metabolism
Female
Flow Cytometry
Follow-Up Studies
Humans
Immunoassay
Lymphoma diagnosis
Male
Middle Aged
Prospective Studies
Retina metabolism
Retinal Neoplasms metabolism
Vitreous Body metabolism
Choroid diagnostic imaging
Choroid Neoplasms diagnosis
Cytokines metabolism
Lymphoma metabolism
Retina diagnostic imaging
Retinal Neoplasms diagnosis
Vitreous Body diagnostic imaging
Subjects
Details
- Language :
- English
- ISSN :
- 1755-3768
- Volume :
- 97
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Acta ophthalmologica
- Publication Type :
- Academic Journal
- Accession number :
- 30688042
- Full Text :
- https://doi.org/10.1111/aos.14036