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Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2019 Apr; Vol. 87, pp. 788-795. Date of Electronic Publication: 2019 Feb 01. - Publication Year :
- 2019
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Abstract
- Haemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species. Among these, PKC-θ (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. To date, no research on immunological functions of fish Pkc-θ has been reported. To address this issue, we cloned the grass carp pkc-θ gene. Phylogenetic and syntenic analysis showed that this gene is the most evolutionarily conserved relative to zebrafish. Real-time quantitative PCR (RT-qPCR) indicated that pkc-θ was expressed at high levels in the gills and spleen of healthy grass carp. Infection with GCRV down regulated pkc-θ expression in the gills and spleen. Gene products that function upstream and downstream of pkc-θ were up regulated in the gill, but were down-regulated in the spleen. These results suggest that direct or indirect targeting of pkc-θ by GCRV may help the virus evade host immune defences in the spleen. Phorbol ester (PMA) treatment of Jurkat T cells induced translocation of grass carp Pkc-θ from the cytoplasm to the plasma membrane. This response to PMA suggests evolutionary conservation of an immune response function in fish Pkc-θ, as well as conservation of its sequence and structural domains. This study expanded our knowledge of the fish PKC gene family, and explored the role of pkc-θ in function of the grass carp immune system, providing new insights which may facilitate further studies of its biological functions.<br /> (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Fish Proteins chemistry
Fish Proteins genetics
Fish Proteins immunology
Gene Expression Profiling veterinary
Protein Kinase C-theta chemistry
Random Allocation
Reoviridae physiology
Reoviridae Infections immunology
Reoviridae Infections veterinary
Sequence Alignment veterinary
Carps genetics
Carps immunology
Fish Diseases immunology
Gene Expression Regulation immunology
Immunity, Innate genetics
Protein Kinase C-theta genetics
Protein Kinase C-theta immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 87
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 30716520
- Full Text :
- https://doi.org/10.1016/j.fsi.2019.01.057