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Therapeutic effects of three human-derived materials in a mouse corneal alkali burn model.
- Source :
-
Cutaneous and ocular toxicology [Cutan Ocul Toxicol] 2019 Dec; Vol. 38 (4), pp. 315-321. Date of Electronic Publication: 2019 Jul 23. - Publication Year :
- 2019
-
Abstract
- Purpose: To compare the therapeutic effects of human derivatives in a mouse alkali burn model. Methods: The right eyes of mice were injured using NaOH. After alkali injury, one of the following agents was topically administered for 7 d: human amniotic membrane (hAM) suspension, human umbilical cord serum (hUCS), and human peripheral blood serum (hPBS), or saline. The epithelial defect areas on days 1, 2, and 3 degrees of opacity on days 2, 3, and 7, and corneal neovascularization (NV) areas on day 7 were evaluated. Histologic examination and mRNA expression levels of tumour necrosis factor (TNF)-α, interleukin (IL)-6, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, MMP-8, and MMP-9 were also evaluated on day 7. Results: The epithelial defect areas in the hUCS group were smaller than those in the control and hPBS groups on day 3 ( p < .05, respectively). The epithelial defect areas in the hAM suspension group showed smaller than those in the control and hPBS groups on days 1 and 2 ( p < .05, respectively). The degrees of opacity were lower in all treatment groups than that of the saline control group on day 7 ( p < .05, respectively). Corneal NV areas were not different among groups on day 7 ( p = 0.20). The expression levels of TNF-α, IL-6, MMP-8, and MMP-9 mRNA and the infiltration of the inflammatory cells in all treatment groups were lesser than those in the control group on day 7 ( p<  .05, respectively). Conclusions: All treatments reduced inflammatory reactions and corneal opacity development. Corneal reepithelialization was faster in the hUCS group.
- Subjects :
- Animals
Burns, Chemical pathology
Cornea drug effects
Cornea pathology
Corneal Neovascularization pathology
Corneal Opacity pathology
Eye Burns chemically induced
Eye Burns pathology
Humans
Male
Mice, Inbred BALB C
Amnion
Burns, Chemical therapy
Corneal Neovascularization therapy
Corneal Opacity therapy
Eye Burns therapy
Serum
Sodium Hydroxide toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1556-9535
- Volume :
- 38
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cutaneous and ocular toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 30741024
- Full Text :
- https://doi.org/10.1080/15569527.2019.1573244