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Cyclin B3 is required for metaphase to anaphase transition in oocyte meiosis I.
- Source :
-
The Journal of cell biology [J Cell Biol] 2019 May 06; Vol. 218 (5), pp. 1553-1563. Date of Electronic Publication: 2019 Feb 15. - Publication Year :
- 2019
-
Abstract
- Meiosis with a single round of DNA replication and two successive rounds of chromosome segregation requires specific cyclins associated with cyclin-dependent kinases (CDKs) to ensure its fidelity. But how cyclins control the distinctive meiosis is still largely unknown. In this study, we explored the role of cyclin B3 in female meiosis by generating Ccnb3 mutant mice via CRISPR/Cas9. Ccnb3 mutant oocytes characteristically arrested at metaphase I (MetI) with normal spindle assembly and lacked enough anaphase-promoting complex/cyclosome (APC/C) activity, which is spindle assembly checkpoint (SAC) independent, to initiate anaphase I (AnaI). Securin siRNA or CDK1 inhibitor supplements rescued the MetI arrest. Furthermore, CCNB3 directly interacts with CDK1 to exert kinase function. Besides, the MetI arrest oocytes had normal development after intracytoplasmic sperm injection (ICSI) or parthenogenetic activation (PA), along with releasing the sister chromatids, which implies that Ccnb3 exclusively functioned in meiosis I, rather than meiosis II. Our study sheds light on the specific cell cycle control of cyclins in meiosis.<br /> (© 2019 Li et al.)
- Subjects :
- Anaphase-Promoting Complex-Cyclosome metabolism
Animals
CDC2 Protein Kinase metabolism
Embryonic Development
Female
M Phase Cell Cycle Checkpoints
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mutation
Oocytes cytology
Anaphase physiology
Chromosome Segregation
Cyclin B physiology
Kinetochores physiology
Meiosis physiology
Metaphase physiology
Oocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1540-8140
- Volume :
- 218
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 30770433
- Full Text :
- https://doi.org/10.1083/jcb.201808088