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Preparative isolation and purification of zearalenone from rice culture by combined use of macroporous resin column and high-speed counter-current chromatography.
- Source :
-
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2019 Mar 15; Vol. 1110-1111, pp. 43-50. Date of Electronic Publication: 2019 Feb 10. - Publication Year :
- 2019
-
Abstract
- Zearalenone is one of the most harmful mycotoxins found in grains and there is a large demand for zearalenone substrate for research purposes. A new separation method was developed for the preparative purification of zearalenone from rice culture of Fusarium graminearum by utilizing macroporous resin column combined with high-speed counter-current chromatography. Zearalenone was adsorpted on XAD-2 resin at 25 °C, neutral pH and a feed flow of 4 BV/h, followed by dynamic desorption by 60% ethanol solution. Further purification was achieved by high-speed counter-current chromatography using an optimized biphasic solvent system. A total of 267 mg of zearalenone crystal was obtained in one single run from 4.2 g of crude extract. The purity of the final product was 98.9% and the total recovery yield of zearalenone in this study was 73.9%. This dual-step purification procedure provided an effective way to obtain the costly mycotoxin for both toxicological and detoxification studies on zearalenone.<br /> (Copyright © 2019. Published by Elsevier B.V.)
Details
- Language :
- English
- ISSN :
- 1873-376X
- Volume :
- 1110-1111
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 30776614
- Full Text :
- https://doi.org/10.1016/j.jchromb.2019.02.009