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Electrochemical paper based cancer biosensor using iron oxide nanoparticles decorated PEDOT:PSS.
- Source :
-
Analytica chimica acta [Anal Chim Acta] 2019 May 16; Vol. 1056, pp. 135-145. Date of Electronic Publication: 2019 Jan 08. - Publication Year :
- 2019
-
Abstract
- We report results of the studies relating to the fabrication of a label-free, flexible, light weight and disposable conducting paper based immunosensing platform comprising of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) and nanostructured iron oxide (nFe <subscript>2</subscript> O <subscript>3</subscript> @PEDOT:PSS) nanocomposite for detection of carcinoembryonic antigen (CEA), a cancer biomarker. The effect of various solvents such as sorbitol, ethanol, propanol, n-methyl-2-pyrrolidone (NMP) and dimethyl sulfoxide (DMSO) on the electrical conductivity of Whatman filter paper (WP) modified with nFe <subscript>2</subscript> O <subscript>3</subscript> @PEDOT:PSS/WP was investigated. The electrical conductivity of the PEDOT:PSS/WP electrode was found to be enhanced by two orders of magnitude (from 6.8× 10 <superscript>-4</superscript> to 1.92 × 10 <superscript>-2</superscript> Scm <superscript>-1</superscript> ) after its treatment with DMSO. Further, nFe <subscript>2</subscript> O <subscript>3</subscript> doped PEDOT:PSS/WP electrode exhibited the electrical conductivity as 2.4 × 10 <superscript>-2</superscript> Scm <superscript>-1</superscript> . Besides this, the incorporation of iron oxide nanoparticles (nFe <subscript>2</subscript> O <subscript>3</subscript> ) into PEDOT:PSS/WP resulted in improved electrochemical performance and signal stability. This nFe <subscript>2</subscript> O <subscript>3</subscript> @PEDOT:PSS/WP based platform was used for immobilization of the anti-carcinoembronic antigen (anti-CEA) protein for quantitative estimation of cancer biomarker (CEA). The results of electrochemical response studies revealed that this conducting paper based immunoelectrode had a sensitivity of 10.2 μAng <superscript>-1</superscript> mLcm <superscript>-2</superscript> in the physiological range (4-25 ngmL <superscript>-1</superscript> ) and shelf life of 34 days. Further, the proposed immunoelectrode was validated with conventional ELISA for the detection of CEA in serum samples of cancer patients.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-4324
- Volume :
- 1056
- Database :
- MEDLINE
- Journal :
- Analytica chimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 30797454
- Full Text :
- https://doi.org/10.1016/j.aca.2018.12.053