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Neuroprotective Effects of Collagen-Glycosaminoglycan Matrix Implantation following Surgical Brain Injury.
- Source :
-
Mediators of inflammation [Mediators Inflamm] 2019 Jan 27; Vol. 2019, pp. 6848943. Date of Electronic Publication: 2019 Jan 27 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Background: Neurological deficits following neurosurgical procedures are inevitable; however, there are still no effective clinical treatments. Earlier reports revealed that collagen-glycosaminoglycan (CG) matrix implantation promotes angiogenesis, neurogenesis, and functional recovery following surgical brain injury (SBI). The present study was conducted to further examine the potential neuroprotective effects of collagen-glycosaminoglycan (CG) matrix implantation following neurosurgery.<br />Methods: CG implantation was performed in the lesion cavity created by surgical trauma. The Sprague-Dawley rat model of SBI was used as established in the previous study by the author. The rats were divided into three groups as follows: (1) sham (SHAM), (2) surgery-induced lesion cavity (L), and (3) CG matrix implantation following surgery-induced lesion cavity (L+CG). Proinflammatory (tumor necrosis factor-alpha (TNF- α ), interleukin-6 (IL-6), and NF- κ B (nuclear factor kappa-light-chain-enhancer of activated B cells)) and anti-inflammatory (IL-10 and granulocyte-macrophage colony-stimulating factor (GMCSF)) cytokine expression was evaluated by enzyme-linked immunosorbent assays. Microglial activation was evaluated by immunohistochemistry, and the neuroprotective effect of CG matrix implantation was evaluated by an immunohistochemical study of microglia ED-1 and IBA-1 (activated microglia) and myeloperoxidase (MPO) and by the analysis of IL-6, IL-10, TNF- α , NF- κ B, and GMCSF cytokine levels. Apoptosis was also assessed using a TUNEL assay.<br />Results: The results showed that CG matrix implantation following surgically induced lesions significantly decreased the density of ED-1, IBA-1, and MPO (activated microglia). The tissue concentration of proinflammatory cytokines, such as TNF- α , IL-6, and NF- κ B was significantly decreased. Conversely, the anti-inflammatory cytokines GMCSF and IL-10 were significantly increased.<br />Conclusions: Implantation of the CG matrix following SBI has neuroprotective effects, including the suppression of microglial activation and the production of inflammatory-related cytokines.
- Subjects :
- Animals
Brain drug effects
Brain surgery
Collagen chemistry
Glycosaminoglycans chemistry
Immunohistochemistry
In Situ Nick-End Labeling
Interleukin-10 metabolism
Male
Microglia drug effects
Microglia metabolism
NF-kappa B metabolism
Rats
Rats, Sprague-Dawley
Signal Transduction drug effects
Tumor Necrosis Factor-alpha metabolism
Brain Injuries drug therapy
Collagen therapeutic use
Cytokines metabolism
Glycosaminoglycans therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1861
- Volume :
- 2019
- Database :
- MEDLINE
- Journal :
- Mediators of inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 30809107
- Full Text :
- https://doi.org/10.1155/2019/6848943