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In vitro antiviral activity of spirotetronate compounds against dengue virus serotype 2.
- Source :
-
The Journal of general and applied microbiology [J Gen Appl Microbiol] 2019 Sep 14; Vol. 65 (4), pp. 197-203. Date of Electronic Publication: 2019 Feb 28. - Publication Year :
- 2019
-
Abstract
- Spirotetronate compounds are polyketide secondary metabolites with diverse biological functions, such as antibacterial, antitumor and antiviral activities. Three pure spirotetronate compounds (2EPS-A, -B, -C) isolated from Actinomadura strain 2EPS showed inhibitory activity against dengue virus serotype 2 (DENV-2). 2EPS-A, -B and -C demonstrated the LC <subscript>50</subscript> values of 11.6, 27.5 and 12.0 μg/ml, respectively, in a test of cytotoxicity to Vero cells. The least cytotoxic, 2EPS-B, was further analyzed for its impact on viral propagation in a cell-based replication assay. At a concentration of 6.25 μg/ml, it could reduce the DENV-2 infection in Vero cells by about 94% when cells infected with DENV-2 were exposed to 2EPS-B, whereas direct treatment of DENV-2 with 2EPS-B at the same concentration prior to subsequent infection to Vero cell yielded no inhibition. 2EPS-A, -B an -C showed strong DENV-2 NS2B-NS3 protease inhibition in an in vitro assay, with IC <subscript>50</subscript> values of 1.94 ± 0.18, 1.47 ± 0.15 and 2.51 ± 0.21 μg/ml, respectively. Therefore, the spirotetronate compounds appear to prevent viral replication and viral assembly by inhibition of the viral protease.
- Subjects :
- Actinobacteria chemistry
Animals
Chlorocebus aethiops
Dengue Virus enzymology
Dengue Virus physiology
Inhibitory Concentration 50
Polyketides chemistry
Protease Inhibitors chemistry
Protease Inhibitors pharmacology
Serogroup
Vero Cells
Virus Replication drug effects
Antiviral Agents pharmacology
Dengue Virus drug effects
Polyketides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1349-8037
- Volume :
- 65
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of general and applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 30814437
- Full Text :
- https://doi.org/10.2323/jgam.2018.10.001