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Simultaneous determination of tandospirone and its active metabolite, 1-[2-pyrimidyl]-piperazine in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.
- Source :
-
Biomedical chromatography : BMC [Biomed Chromatogr] 2019 Jul; Vol. 33 (7), pp. e4525. Date of Electronic Publication: 2019 Apr 22. - Publication Year :
- 2019
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Abstract
- A rapid, sensitive and selective liquid chromatography-tandem mass spectrometry method for the detection of tandospirone (TDS) and its active metabolite 1-[2-pyrimidyl]-piperazine (1-PP) in Sprague-Dawley rat plasma is described. It was employed in a pharmacokinetic study. These analytes and the internal standards were extracted from plasma using protein precipitation with acetonitrile, then separated on a CAPCELL PAK ADME C <subscript>18</subscript> column using a mobile phase of acetonitrile and 5 mm ammonium formate acidified with formic acid (0.1%, v/v) at a total flow rate of 0.4 mL/min. The detection was performed with a tandem mass spectrometer equipped with an electrospray ionization source. The method was validated to quantify the concentration ranges of 1.000-500.0 ng/mL for TDS and 10.00-500.0 ng/mL for 1-PP. Total time for each chromatograph was 3.0 min. The intra-day precision was between 1.42 and 6.69% and the accuracy ranged from 95.74 to 110.18% for all analytes. Inter-day precision and accuracy ranged from 2.47 to 6.02% and from 98.37 to 105.62%, respectively. The lower limits of quantification were 1.000 ng/mL for TDS and 10.00 ng/mL for 1-PP. This method provided a fast, sensitive and selective analytical tool for quantification of tandospirone and its metabolite 1-PP in plasma necessary for the pharmacokinetic investigation.<br /> (© 2019 John Wiley & Sons, Ltd.)
- Subjects :
- Animals
Buspirone blood
Buspirone chemistry
Buspirone pharmacokinetics
Drug Stability
Female
Isoindoles chemistry
Isoindoles pharmacokinetics
Limit of Detection
Linear Models
Male
Piperazines chemistry
Piperazines pharmacokinetics
Pyrimidines chemistry
Pyrimidines pharmacokinetics
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Buspirone analogs & derivatives
Chromatography, High Pressure Liquid methods
Isoindoles blood
Piperazines blood
Pyrimidines blood
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0801
- Volume :
- 33
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biomedical chromatography : BMC
- Publication Type :
- Academic Journal
- Accession number :
- 30822365
- Full Text :
- https://doi.org/10.1002/bmc.4525