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Hacking the Cancer Genome: Profiling Therapeutically Actionable Long Non-coding RNAs Using CRISPR-Cas9 Screening.
- Source :
-
Cancer cell [Cancer Cell] 2019 Apr 15; Vol. 35 (4), pp. 545-557. Date of Electronic Publication: 2019 Feb 28. - Publication Year :
- 2019
-
Abstract
- Long non-coding RNAs (lncRNAs) represent a huge reservoir of potential cancer targets. Such "onco-lncRNAs" have resisted traditional RNAi methods, but CRISPR-Cas9 genome editing now promises functional screens at high throughput and low cost. The unique biology of lncRNAs demands screening strategies distinct from protein-coding genes. The first such screens have identified hundreds of onco-lncRNAs promoting cell proliferation and drug resistance. Ongoing developments will further improve screen performance and translational relevance. This Review aims to highlight the potential of CRISPR screening technology for discovering new onco-lncRNAs, and to guide molecular oncologists wishing to apply it to their cancer of interest.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antineoplastic Agents therapeutic use
Biomarkers, Tumor metabolism
CRISPR-Associated Protein 9 metabolism
Cell Proliferation drug effects
Cell Proliferation genetics
Drug Resistance, Neoplasm genetics
Gene Expression Regulation, Neoplastic
Humans
Molecular Targeted Therapy
Neoplasms drug therapy
Neoplasms metabolism
Neoplasms pathology
RNA, Long Noncoding metabolism
Biomarkers, Tumor genetics
CRISPR-Associated Protein 9 genetics
CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats
High-Throughput Nucleotide Sequencing methods
Neoplasms genetics
RNA, Long Noncoding genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3686
- Volume :
- 35
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cancer cell
- Publication Type :
- Academic Journal
- Accession number :
- 30827888
- Full Text :
- https://doi.org/10.1016/j.ccell.2019.01.019