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Construction of a dietary-cure Saccharomyces cerevisiae expressing long-acting glucagon-like peptide-1 and investigation of its hypoglycemic activity in type 2 diabetes mellitus mouse model.
- Source :
-
Journal of biosciences [J Biosci] 2019 Mar; Vol. 44 (1). - Publication Year :
- 2019
-
Abstract
- Bio-drug is a new type of beneficial biology expressing therapeutic peptides (protein) as orally administrated medicine to treat diseases, in particular, chronic diseases like diabetes. In order to develop recombinant yeast strains as a bio-drug which could effectively ameliorate type 2 diabetes, an integrating expression vector pNK1-PGK that could successfully express green fluorescent protein (GFP) in Saccharomyces cerevisiae was constructed to demonstrate the normality of the function. A pNK1-PGK vector, which expresses 10 tandem repeats of long-acting glucagon-like peptide-1(10laGLP-1), was cloned and then transformed into the S. cerevisiae INVSc1. The long-acting GLP-1 hypoglycemic yeast (LHY168) that grew rapidly and expressed 10laGLP-1 stably was screened by uracil-deficient plates and Western blot. The expression quantity of 10laGLP-1 reached 1.56 mg/g cell wet weight. Moreover, the oral administration of LHY168 significantly declined the blood glucose in type 2 diabetic mice that were constructed through co-induction of streptozotocin (STZ) and high-fat and high-sugar diet.
- Subjects :
- Animals
Blood Glucose drug effects
Diabetes Mellitus, Experimental pathology
Diabetes Mellitus, Type 2 genetics
Diabetes Mellitus, Type 2 microbiology
Disease Models, Animal
Gene Expression Regulation
Genetic Vectors administration & dosage
Glucagon-Like Peptide 1 administration & dosage
Humans
Mice
Peptides genetics
Saccharomyces cerevisiae genetics
Tandem Repeat Sequences genetics
Diabetes Mellitus, Experimental drug therapy
Diabetes Mellitus, Type 2 drug therapy
Glucagon-Like Peptide 1 genetics
Hypoglycemic Agents administration & dosage
Peptides administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0973-7138
- Volume :
- 44
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biosciences
- Publication Type :
- Academic Journal
- Accession number :
- 30837360