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Femtosecond laser induced densification within cell-laden hydrogels results in cellular alignment.
- Source :
-
Biofabrication [Biofabrication] 2019 Apr 05; Vol. 11 (3), pp. 035005. Date of Electronic Publication: 2019 Apr 05. - Publication Year :
- 2019
-
Abstract
- The unique capabilities of ultrafast lasers to introduce user-defined microscale modifications within 3D cell-laden hydrogels have been used to investigate fundamental cellular phenomenon such as adhesion, alignment, migration and organization. In this work, we report a new material modification phenomenon coined as 'densification' and its influence on the behavior of encapsulated cells. Femtosecond laser writing technique was used to write densified lines of width 1-5 μm within the bulk of gelatin methacrylate (GelMA) constructs. We found that densified micro-lines within cell-laden GelMA constructs resulted in preferential and localized alignment of encapsulated human endothelial cells. Degree of cellular alignment was characterized as a function of cell-culture time and the spacing between the densified line patterns. This phenomenon was found to be true for several cell lines, including mouse fibroblasts and osteocytes, and mesenchymal stem cells derived from human induced pluripotent cells. This first report of physical densification using fs lasers can be potentially extended for investigating cell behavior within other photosensitive hydrogels.
- Subjects :
- Animals
Cross-Linking Reagents chemistry
Fibroblasts cytology
Fluorescence
Gelatin pharmacology
Human Umbilical Vein Endothelial Cells cytology
Human Umbilical Vein Endothelial Cells drug effects
Human Umbilical Vein Endothelial Cells metabolism
Humans
Induced Pluripotent Stem Cells cytology
Methacrylates pharmacology
Mice
Sus scrofa
Time Factors
Hydrogels pharmacology
Lasers
Subjects
Details
- Language :
- English
- ISSN :
- 1758-5090
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biofabrication
- Publication Type :
- Academic Journal
- Accession number :
- 30865942
- Full Text :
- https://doi.org/10.1088/1758-5090/ab0f8b