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The Effects of β-Lactam Antibiotics on Surface Modifications of Multidrug-Resistant Escherichia coli: A Multiscale Approach.
- Source :
-
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [Microsc Microanal] 2019 Feb; Vol. 25 (1), pp. 135-150. - Publication Year :
- 2019
-
Abstract
- Possible multidrug-resistant (MDR) mechanisms of four resistant strains of Escherichia coli to a model β-lactam, ampicillin, were investigated using contact angle measurements of wettability, crystal violet assays of permeability, biofilm formation, fluorescence imaging, and nanoscale analyses of dimensions, adherence, and roughness. Upon exposure to ampicillin, one of the resistant strains, E. coli A5, changed its phenotype from elliptical to spherical, maintained its roughness and biofilm formation abilities, decreased its length and surface area, maintained its cell wall integrity, increased its hydrophobicity, and decreased its nanoscale adhesion to a model surface of silicon nitride. Such modifications are suggested to allow these cells to conserve energy during metabolic dormancy. In comparison, resistant strains E. coli D4, A9, and H5 elongated their cells, increased their roughness, increased their nanoscale adhesion forces, became more hydrophilic, and increased their biofilm formation upon exposure to ampicillin. These results suggest that these strains resisted ampicillin through biofilm formation that possibly introduces diffusion limitations to antibiotics. Investigations of how MDR bacterial cells modify their surfaces in response to antibiotics can guide research efforts aimed at designing more effective antibiotics and new treatment strategies for MDR bacterial infections.
- Subjects :
- Ampicillin pharmacology
Bacterial Adhesion drug effects
Biofilms drug effects
Biofilms growth & development
Cell Membrane Permeability drug effects
Cell Wall drug effects
Escherichia coli cytology
Escherichia coli growth & development
Escherichia coli metabolism
Gelatin
Hydrophobic and Hydrophilic Interactions drug effects
Immobilization
Microbial Sensitivity Tests
Phenotype
Surface Properties drug effects
Wettability drug effects
Anti-Bacterial Agents pharmacology
Drug Resistance, Multiple, Bacterial drug effects
Escherichia coli drug effects
beta-Lactams pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1435-8115
- Volume :
- 25
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
- Publication Type :
- Academic Journal
- Accession number :
- 30869575
- Full Text :
- https://doi.org/10.1017/S1431927618015696