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Assessment and characterization of the bacterial community structure in advanced activated sludge systems.
- Source :
-
Bioresource technology [Bioresour Technol] 2019 Jun; Vol. 282, pp. 254-261. Date of Electronic Publication: 2019 Mar 06. - Publication Year :
- 2019
-
Abstract
- The present study is aimed to assess and characterize the structure of bacterial community in advanced activated sludge systems. In particular, activated sludge samples were collected from an Integrated Fixed-film Activated Sludge - Membrane Bioreactor pilot plant under a University of Cape Town configuration with in-series anaerobic (Noair)/anoxic (Anox)/aerobic (Oxy) reactors - and further analyzed. The achieved results - based on Next Generation Sequencing (NGS) of 16S rDNA amplicons - revealed that the bacterial biofilm (bf) communities on plastic carriers of Oxy and Anox reactors had a greater diversity compared to suspended (sp) bacterial flocs of Oxy, Anox and Noair. Indeed, the Shannon diversity indices of both biofilm communities were higher than those of suspended growth samples (Oxy-bf = 4.1 and Anox-bf = 4.2 vs. Oxy-sp = 3.4, Anox-sp = 3.5 and Noair-sp = 3.4). The most striking differences have been reported in Rhodobacteraceae being more abundant in biofilm specimens than in suspended biomass samples. The vast majority of the identified bacteria differs from those obtained by culture dependent method, thus suggesting that NGS-based method is really suitable to analyze the bacterial community composition, even in advanced systems for wastewater treatment.<br /> (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Subjects :
- Biofilms
Biomass
Bioreactors microbiology
Microbiota
Sewage microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 282
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 30870691
- Full Text :
- https://doi.org/10.1016/j.biortech.2019.03.018