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Berberine attenuates sodium palmitate-induced lipid accumulation, oxidative stress and apoptosis in grass carp(Ctenopharyngodon idella)hepatocyte in vitro.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2019 May; Vol. 88, pp. 518-527. Date of Electronic Publication: 2019 Mar 14. - Publication Year :
- 2019
-
Abstract
- The objective of this work was to investigate the effect of berberine (BBR) on the Cell viability, lipid accumulation, apoptosis, cytochrome c, caspase-9 and caspase-3 in lipid accumulation-hepatocytes induced by sodium palmitate in vitro. The lipid accumulation-hepatocytes (induced by 0.5 mM sodium palmitate for 24 h) were treated with 5 μM berberine for 12 h. Then, the Cell viability, intracellular triglyceride (TG) content, lipid peroxide (LPO), malonaldehyde (MDA) content, cytochrome c, caspase-9, caspase-3 and apoptosis were detected. Sodium palmitate decreased Cell viability and increased intracellular TG content, lipid droplet accumulation, LPO and MDA concentrations, caused caspase-3 and caspase-9 activation, then led to apoptosis accompanied by cytochrome c release from mitochondria into the cytoplasm. Beberine could improve intracellular lipid droplet accumulation and oxidative stress, while reduce apoptosis induced by sodium palmitate.<br /> (Copyright © 2019. Published by Elsevier Ltd.)
- Subjects :
- Animals
Caspases metabolism
Cell Survival drug effects
Cells, Cultured
Cytochromes c metabolism
Mitochondria drug effects
Mitochondria metabolism
Triglycerides metabolism
Apoptosis drug effects
Berberine pharmacology
Carps
Hepatocytes drug effects
Lipid Metabolism drug effects
Oxidative Stress drug effects
Palmitic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 88
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 30880233
- Full Text :
- https://doi.org/10.1016/j.fsi.2019.02.055