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Evaluation of preimplantation genetic testing based on next-generation sequencing for balanced reciprocal translocation carriers.
- Source :
-
Reproductive biomedicine online [Reprod Biomed Online] 2019 May; Vol. 38 (5), pp. 669-675. Date of Electronic Publication: 2019 Jan 08. - Publication Year :
- 2019
-
Abstract
- Research Question: Can next-generation sequencing (NGS) based on copy number variation sequencing (CNV-Seq) identify normal/balanced embryos in balanced reciprocal translocation carriers and what are their reproductive outcomes?<br />Design: One hundred couples with balanced reciprocal translocation who underwent a total of 134 preimplantation genetic testing (PGT) cycles between January 2015 and October 2017 were evaluated. Trophectoderm cells of blastocysts were biopsied for CNV-Seq-based NGS. All the balanced/normal blastocysts were vitrified and cryopreserved. Single balanced/normal blastocysts were warmed and transferred in the subsequent frozen embryo transfer (FET) cycle.<br />Results: During the study period, 400 blastocysts were analysed by NGS-PGT, of which 109 (27.25%) were balanced and euploid. A total of 52 blastocysts were transferred in the FET cycle. Clinical pregnancy was confirmed in 34 women (65.38%), with a miscarriage rate of 2.94%; 26 healthy term babies were born, including 24 singletons and one set of twins, while eight couples had ongoing pregnancies. Amniocentesis revealed a fetal chromosome status that was consistent with the NGS-PGT results. Female carriers had a significantly higher blastocyst rate than did the male carriers (37.01% versus 31.27%, P = 0.04). The transferable blastocyst rate was higher in couples treated with gonadotrophin-releasing hormone (GnRH) antagonist than in those treated with GnRH agonist (38.20% versus 24.37%, P = 0.01). However, neither carrier sex nor ovarian stimulation protocol influenced the clinical pregnancy rate.<br />Conclusions: CNV-Seq-based NGS is an efficient and reliable PGT method for balanced reciprocal translocation.<br /> (Copyright © 2019 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1472-6491
- Volume :
- 38
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Reproductive biomedicine online
- Publication Type :
- Academic Journal
- Accession number :
- 30885668
- Full Text :
- https://doi.org/10.1016/j.rbmo.2018.12.043