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Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment.
- Source :
-
BMC microbiology [BMC Microbiol] 2019 Mar 19; Vol. 19 (1), pp. 62. Date of Electronic Publication: 2019 Mar 19. - Publication Year :
- 2019
-
Abstract
- Background: Nontuberculous mycobacteria (NTM) are ubiquitous in nature and recognized agents of opportunistic infection, which is often aggravated by their intrinsic resistance to antimicrobials, poorly defined therapeutic strategies and by the lack of new drugs. However, evaluation of their prevalence in anthropogenic environments and the associated antimicrobial resistance profiles have been neglected. In this work, we sought to determine minimal inhibitory concentrations of 25 antimicrobials against 5 NTM isolates recovered from a tertiary-care hospital surfaces. Antimicrobial susceptibilities of 5 other Corynebacterineae isolated from the same hospital were also determined for their potential clinical relevance.<br />Results: Our phylogenetic study with each of the NTM isolates confirm they belong to Mycobacterium obuense, Mycobacterium mucogenicum and Mycobacterium paragordonae species, the latter initially misidentified as strains of M. gordonae, a species frequently isolated from patients with NTM disease in Portugal. In contrast to other strains, the M. obuense and M. mucogenicum examined here were resistant to several of the CLSI-recommended drugs, suggestive of multidrug-resistant profiles. Surprisingly, M. obuense was susceptible to vancomycin. Their genomes were sequenced allowing detection of gene erm (erythromycin resistance methylase) in M. obuense, explaining its resistance to clarithromycin. Remarkably, and unlike other strains of the genus, the Corynebacterium isolates were highly resistant to penicillin, ciprofloxacin and linezolid.<br />Conclusions: This study highlights the importance of implementing effective measures to screen, accurately identify and control viable NTM and closely related bacteria in hospital settings. Our report on the occurrence of rare NTM species with antibiotic susceptibility profiles that are distinct from those of the corresponding Type strains, along with unexpected resistance mechanisms detected seem to suggest that resistance may be more common than previously thought and also a potential threat to frail and otherwise vulnerable inpatients.
- Subjects :
- Corynebacterium drug effects
Equipment and Supplies, Hospital microbiology
Humans
Microbial Sensitivity Tests
Mycobacterium Infections, Nontuberculous microbiology
Patients' Rooms
Phylogeny
Portugal
Tertiary Care Centers statistics & numerical data
Anti-Bacterial Agents pharmacology
Cross Infection microbiology
Drug Resistance, Multiple, Bacterial
Nontuberculous Mycobacteria drug effects
Nontuberculous Mycobacteria isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2180
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 30890149
- Full Text :
- https://doi.org/10.1186/s12866-019-1428-4