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Effects of lactulose on renal function and gut microbiota in adenine-induced chronic kidney disease rats.
- Source :
-
Clinical and experimental nephrology [Clin Exp Nephrol] 2019 Jul; Vol. 23 (7), pp. 908-919. Date of Electronic Publication: 2019 Mar 20. - Publication Year :
- 2019
-
Abstract
- Background: Constipation is frequently observed in patients with chronic kidney disease (CKD). Lactulose is expected to improve the intestinal environment by stimulating bowel movements as a disaccharide laxative and prebiotic. We studied the effect of lactulose on renal function in adenine-induced CKD rats and monitored uremic toxins and gut microbiota.<br />Methods: Wistar/ST male rats (10-week-old) were fed 0.75% adenine-containing diet for 3 weeks to induce CKD. Then, they were divided into three groups and fed as follows: control, normal diet; and 3.0- and 7.5-Lac, 3.0% and 7.5% lactulose-containing diets, respectively, for 4 weeks. Normal diet group was fed normal diet for 7 weeks. The rats were observed for parameters including renal function, uremic toxins, and gut microbiota.<br />Results: The control group showed significantly higher serum creatinine (sCr) and blood urea nitrogen (BUN) 3 weeks after adenine feeding than at baseline, with a 8.5-fold increase in serum indoxyl sulfate (IS). After switching to 4 weeks of normal diet following adenine feeding, the sCr and BUN in control group remained high with a further increase in serum IS. In addition, tubulointerstitial fibrosis area was increased in control group. On the other hand, 3.0- and 7.5-Lac groups improved sCr and BUN levels, and suppressed tubulointerstitial fibrosis, suggesting preventing of CKD progression by lactulose. Lac groups also lowered level of serum IS and proportions of gut microbiota producing IS precursor.<br />Conclusion: Lactulose modifies gut microbiota and ameliorates CKD progression by suppressing uremic toxin production.
- Subjects :
- Animals
Bacteria metabolism
Biomarkers blood
Blood Urea Nitrogen
Creatinine blood
Disease Models, Animal
Disease Progression
Fibrosis
Kidney metabolism
Kidney pathology
Kidney physiopathology
Male
Oxidative Stress drug effects
Rats, Wistar
Renal Insufficiency, Chronic chemically induced
Renal Insufficiency, Chronic microbiology
Renal Insufficiency, Chronic physiopathology
Uremia chemically induced
Uremia microbiology
Uremia physiopathology
Adenine
Bacteria drug effects
Gastrointestinal Microbiome drug effects
Kidney drug effects
Lactulose pharmacology
Prebiotics
Renal Insufficiency, Chronic prevention & control
Uremia prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1437-7799
- Volume :
- 23
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Clinical and experimental nephrology
- Publication Type :
- Academic Journal
- Accession number :
- 30895529
- Full Text :
- https://doi.org/10.1007/s10157-019-01727-4