Back to Search
Start Over
Transcription termination sequences support the expression of transgene product secreted with milk.
- Source :
-
Transgenic research [Transgenic Res] 2019 Aug; Vol. 28 (3-4), pp. 401-410. Date of Electronic Publication: 2019 Mar 28. - Publication Year :
- 2019
-
Abstract
- Expression of the reporter gene in transgenic animals depends on the surrounding chromatin environment. Recent genome-wide studies have shown that, in mammals, the entire genome is transcribed. Transcription through a transgene often has a negative effect on the expression of a reporter gene. Here, we compared the ability of well-studied chicken chromatin insulator HS4 and bidirectional transcription terminators from the human genome to support high-level expression of the firefly luciferase gene (Fluc) under control of the previously characterized goat β-casein gene promoter. The insertion of HS4 or either of the two transcription terminators upstream of the promoter resulted in tenfold enhancement of Fluc expression in the mammary glands of transgenic mice. These results suggest that transcriptional terminators, similar to the HS4 insulator, can be used to improve the reporter gene expression in transgenic animals.
- Subjects :
- Animals
Chickens
Female
Genetic Vectors
Goats
Humans
Insulator Elements
Luciferases, Firefly genetics
Mice
Mice, Transgenic
Transgenes genetics
Caseins genetics
Luciferases, Firefly metabolism
Mammary Glands, Animal metabolism
Milk metabolism
Promoter Regions, Genetic
Transcription Termination, Genetic
Transgenes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-9368
- Volume :
- 28
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Transgenic research
- Publication Type :
- Academic Journal
- Accession number :
- 30919251
- Full Text :
- https://doi.org/10.1007/s11248-019-00122-9