Back to Search
Start Over
Efficient Conversion of Cane Molasses Towards High-Purity Isomaltulose and Cellular Lipid Using an Engineered Yarrowia lipolytica Strain in Fed-Batch Fermentation.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2019 Mar 28; Vol. 24 (7). Date of Electronic Publication: 2019 Mar 28. - Publication Year :
- 2019
-
Abstract
- : Cane molasses is one of the main by-products of sugar refineries, which is rich in sucrose. In this work, low-cost cane molasses was introduced as an alternative substrate for isomaltulose production. Using the engineered Yarrowia lipolytica, the isomaltulose production reached the highest (102.6 g L <superscript>-</superscript> ¹) at flask level with pretreated cane molasses of 350 g L <superscript>-</superscript> ¹ and corn steep liquor of 1.0 g L <superscript>-</superscript> ¹. During fed-batch fermentation, the maximal isomaltulose concentration (161.2 g L <superscript>-</superscript> ¹) was achieved with 0.96 g g <superscript>-</superscript> ¹ yield within 80 h. Simultaneously, monosaccharides were completely depleted, harvesting the high isomaltulose purity (97.4%) and high lipid level (12.2 g L <superscript>-</superscript> ¹). Additionally, the lipids comprised of 94.29% C <subscript>16</subscript> and C <subscript>18</subscript> fatty acids, were proved suitable for biodiesel production. Therefore, the bioprocess employed using cane molasses in this study was low-cost and eco-friendly for high-purity isomaltulose production, coupling with valuable lipids.
- Subjects :
- Biofuels
Biotransformation drug effects
Carbon pharmacology
Fatty Acids analysis
Isomaltose isolation & purification
Lipids biosynthesis
Yarrowia drug effects
Batch Cell Culture Techniques methods
Fermentation drug effects
Genetic Engineering methods
Isomaltose analogs & derivatives
Lipids chemistry
Molasses
Saccharum chemistry
Yarrowia metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 24
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 30925836
- Full Text :
- https://doi.org/10.3390/molecules24071228