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miR-1246 as a therapeutic target in oral submucosa fibrosis pathogenesis.
miR-1246 as a therapeutic target in oral submucosa fibrosis pathogenesis.
- Source :
-
Journal of the Formosan Medical Association = Taiwan yi zhi [J Formos Med Assoc] 2019 Jul; Vol. 118 (7), pp. 1093-1098. Date of Electronic Publication: 2019 Mar 28. - Publication Year :
- 2019
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Abstract
- Background/purpose: Oral submucous fibrosis (OSF) is a precancerous condition of oral cancer with a complex etiology. Our previous work has demonstrated that non-coding RNA miR-1246 contributes to the cancer stemness of oral cancer. In the current study, we sought to investigate the effect of the inhibition of miR-1246 on the oral fibrogenesis.<br />Methods: The expression levels of miR-1246 in OSF tissues and fibrotic buccal mucosal fibroblasts (fBMFs) were examined by qRT-PCR. Collagen gel contraction and migration assays were conducted to evaluate the myofibroblast activities. The relationship between miR-1246 and type I collagen was assessed and the protein expression of type I collagen was determined by Western blot.<br />Results: MiR-1246 expression was upregulated in both OSF specimen and fBMFs compared to the normal counterparts. Inhibition of miR-1246 successfully suppressed the myofibroblast activities, including collagen gel contractility and migration capacity. Moreover, the expression of miR-1246 was positively correlated with type I collagen and the expression of type I collagen was abrogated by repression of miR-1246.<br />Conclusion: MiR-1246 is not only critical to the maintenance of oral stemness but also important to the activation of myofibroblasts. Our results showed that miR-1246 is positively associated with the type I collagen, which may be a downstream effector of miR-1246 and responsible for the fibrosis effect on fBMFs.<br /> (Copyright © 2019. Published by Elsevier B.V.)
Details
- Language :
- English
- ISSN :
- 0929-6646
- Volume :
- 118
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of the Formosan Medical Association = Taiwan yi zhi
- Publication Type :
- Academic Journal
- Accession number :
- 30928188
- Full Text :
- https://doi.org/10.1016/j.jfma.2019.02.014