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Stability of the Metabolite Signature Resulting from the MIPS1 Mutation in Low Phytic Acid Soybean ( Glycine max L. Merr.) Mutants upon Cross-Breeding.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2019 May 01; Vol. 67 (17), pp. 5043-5052. Date of Electronic Publication: 2019 Apr 19. - Publication Year :
- 2019
-
Abstract
- The low phytic acid ( lpa) soybean ( Glycine max L. Merr.) mutant Gm-lpa-TW-1-M, resulting from a 2 bp deletion in GmMIPS1, was crossed with a commercial cultivar. F <subscript>3</subscript> and F <subscript>5</subscript> progenies were subjected to nontargeted GC-based metabolite profiling, allowing analysis of a broad array of low molecular weight constituents. In the homozygous lpa mutant progenies the intended phytic acid reduction was accompanied by remarkable metabolic changes of nutritionally relevant constituents such as reduced contents of raffinose oligosaccharides and galactosyl cyclitols as well as increased concentrations in sucrose and various free amino acids. The mutation-induced metabolite signature was nearly unaffected by the cross-breeding and consistently expressed over generations and in different growing seasons. Therefore, not only the primary MIPS1 lpa mutant but also its progenies might be valuable genetic resources for commercial breeding programs to produce soybean seeds stably exhibiting improved phytate-related and nutritional properties.
- Subjects :
- Arabidopsis Proteins metabolism
Homozygote
Hybridization, Genetic
Mutation
Myo-Inositol-1-Phosphate Synthase metabolism
Oligosaccharides analysis
Oligosaccharides metabolism
Phytic Acid metabolism
Plant Breeding
Plant Proteins metabolism
Raffinose analysis
Raffinose metabolism
Glycine max chemistry
Glycine max genetics
Glycine max metabolism
Sucrose analysis
Sucrose metabolism
Arabidopsis Proteins genetics
Myo-Inositol-1-Phosphate Synthase genetics
Phytic Acid analysis
Plant Proteins genetics
Glycine max enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 67
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 30977368
- Full Text :
- https://doi.org/10.1021/acs.jafc.9b00817