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Transcriptome analysis of Burkholderia pseudomallei SCV reveals an association with virulence, stress resistance and intracellular persistence.
- Source :
-
Genomics [Genomics] 2020 Jan; Vol. 112 (1), pp. 501-512. Date of Electronic Publication: 2019 Apr 10. - Publication Year :
- 2020
-
Abstract
- Differences in expression of potential virulence and survival genes were associated with B. pseudomallei colony morphology variants. Microarray was used to investigate B. pseudomallei transcriptome alterations among the wild type and small colony variant (SCV) pre- and post-exposed to A549 cells. SCV pre- and post-exposed have lower metabolic requirements and consume lesser energy than the wild type pre- and post-exposed to A549. However, both the wild type and SCV limit their metabolic activities post- infection of A549 cells and this is indicated by the down-regulation of genes implicated in the metabolism of amino acids, carbohydrate, lipid, and other amino acids. Many well-known virulence and survival factors, including T3SS, fimbriae, capsular polysaccharides and stress response were up-regulated in both the wild type and SCV pre- and post-exposed to A549 cells. Microarray analysis demonstrated essential differences in bacterial response associated with virulence and survival pre- and post-exposed to A549 cells.<br /> (Copyright © 2019. Published by Elsevier Inc.)
- Subjects :
- A549 Cells
Apoptosis
Burkholderia pseudomallei drug effects
Burkholderia pseudomallei metabolism
Drug Resistance, Bacterial genetics
Gene Expression Profiling
Humans
Microbial Viability
RNA, Bacterial isolation & purification
Stress, Physiological genetics
Virulence
Virulence Factors genetics
Burkholderia pseudomallei genetics
Burkholderia pseudomallei pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8646
- Volume :
- 112
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 30980902
- Full Text :
- https://doi.org/10.1016/j.ygeno.2019.04.002