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PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer.
- Source :
-
Biological research [Biol Res] 2019 Apr 17; Vol. 52 (1), pp. 24. Date of Electronic Publication: 2019 Apr 17. - Publication Year :
- 2019
-
Abstract
- Background: To analyze the relative expression of PELI3 and its mechanistic involvement in the non-small cell lung cancer (NSCLC).<br />Methods: PELI3 expression in NSCLC tissue samples was determined by the immunohistochemistry. The transcripts abundance of PELI3 was measured with real-time PCR. The protein intensity was analyzed by western blot. The overall survival in respect to PELI3 or miR-365a-5p expression was plotted by the Kaplan-Meier's analysis. Cell growth was determined by colony formation assay. Cell viability was measured by MTT assay. The migration and invasion were evaluated by wound healing and transwell assay respectively. The regulatory effect of miR-365a-5p on PELI3 was interrogated with luciferase reporter assay. The direct binding between miR-365a-5p and PELI3 was analyzed by pulldown assay.<br />Results: PELI3 was aberrantly up-regulated in NSCLC both in vivo and in vitro. High level of PELI3 associated with poor prognosis. PELI3-deficiency significantly inhibited cell viability, colony formation, migration and invasion. We further identified that miR-365a-5p negatively regulated PELI3 in this disease. Ectopic expression of miR-365a-5p in both A549 and H1299 phenocopied PELI3-deficiency. Meanwhile, PELI3-silencing significantly abolished the pro-tumoral effect elicited by miR-365a-5p inhibition.<br />Conclusion: Our results highlighted the importance of dysregulated miR-365a-5p-PELI3 signaling axis in NSCLC.
- Subjects :
- Animals
Antineoplastic Agents pharmacology
Antineoplastic Agents therapeutic use
Carcinoma, Non-Small-Cell Lung drug therapy
Carcinoma, Non-Small-Cell Lung pathology
Cell Line, Tumor
Coloring Agents
Disease Models, Animal
Humans
Lung Neoplasms drug therapy
Lung Neoplasms pathology
MicroRNAs therapeutic use
Tetrazolium Salts
Thiazoles
Ubiquitin-Protein Ligases pharmacology
Carcinoma, Non-Small-Cell Lung metabolism
Down-Regulation physiology
Lung Neoplasms metabolism
MicroRNAs metabolism
Ubiquitin-Protein Ligases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0717-6287
- Volume :
- 52
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biological research
- Publication Type :
- Academic Journal
- Accession number :
- 30995936
- Full Text :
- https://doi.org/10.1186/s40659-019-0230-y