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Oligonucleotide Primers with G 8AE -Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1973, pp. 281-297. - Publication Year :
- 2019
-
Abstract
- We developed a new technique suitable for improved detection of low-copy dsRNA using modified oligonucleotides as primers in RT-qPCR. Insertion of G <superscript>8AE</superscript> -clamp residues into primers significantly improves thermal stability of duplexes with RNA without decrease of hybridization selectivity. The applicability of modified primers is demonstrated for detection of low-copy Kemerovo virus dsRNA.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1973
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 31016709
- Full Text :
- https://doi.org/10.1007/978-1-4939-9216-4_18