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Biological variation of peripheral blood T-lymphocytes.
- Source :
-
Journal of immunological methods [J Immunol Methods] 2019 Jul; Vol. 470, pp. 1-5. Date of Electronic Publication: 2019 Apr 26. - Publication Year :
- 2019
-
Abstract
- Background: Flow cytometric analysis of the lymphocyte subsets has become one of the most commonly used techniques in the routine clinical laboratory. It is frequently used in monitoring lymphocyte recovery after hematopoietic stem cell transplantation (HSCT), as well as diagnosis and treatment of acquired immunodeficiency syndrome (AIDS). Reliable biological variation (BV) data is needed for safe clinical application of these tests. In this study, similar preanalytical and analytical protocols to the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) checklist were followed and a stringent statistical approach was applied to define BV of T-lymphocytes.<br />Methods: During the 10 weeks study period, weekly blood samples were obtained from 30 healthy individuals (20 females, 10 males) and analyzed with Facs Canto (BD Biosciences, San Jose, CA, USA) analyzer using 4-colour BD Multitest CD3/CD8/CD45/CD4 reagents. Data were assessed in terms of normality, tendencies, outliers and variance homogeneity prior to applying coefficient of variance (CV)- analysis of variance (ANOVA) test. Sex-stratified within-individual (CV <subscript>I</subscript> ) and between-individual (CV <subscript>G</subscript> ) BV estimates of CD3+, CD3 + CD4+, CD3 + CD8+, and CD3 + CD4 + CD8+ T lymphocytes were calculated.<br />Results: No difference was found between males and females. Except for the CD3 + CD4 + CD8+ subset, stable BV was found for CD3+, CD3 + CD4+, and CD3 + CD8+ subsets.<br />Conclussion: Instead of using the conventional reference ranges of CD3+, CD3 + CD4+ and CD3 + CD8+ counts for monitoring HIV positive or post-HSCT patients, RCV should be used. Because individualityis characteristic of lymphocytes subsets RCVs should be used instead of RIs for patient monitoring.<br /> (Published by Elsevier B.V.)
- Subjects :
- Adult
Antigens, CD classification
Antigens, CD immunology
Biomarkers analysis
Female
Gene Expression
Genetic Variation
Healthy Volunteers
Humans
Lymphocyte Count
Lymphocyte Subsets cytology
Lymphocyte Subsets metabolism
Male
Middle Aged
Reference Values
Antigens, CD genetics
Flow Cytometry standards
Immunophenotyping standards
Lymphocyte Subsets classification
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7905
- Volume :
- 470
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 31022389
- Full Text :
- https://doi.org/10.1016/j.jim.2019.04.002