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A proteomic comparison of excretion/secretion products in Fasciola hepatica newly excysted juveniles (NEJ) derived from Lymnaea viatrix or Pseudosuccinea columella.
- Source :
-
Experimental parasitology [Exp Parasitol] 2019 Jun; Vol. 201, pp. 11-20. Date of Electronic Publication: 2019 Apr 22. - Publication Year :
- 2019
-
Abstract
- The characteristics of parasitic infections are often tied to host behavior. Although most studies have investigated definitive hosts, intermediate hosts can also play a role in shaping the distribution and accumulation of parasites. This is particularly relevant in larval stages, where intermediate host's behavior could potentially interfere in the molecules secreted by the parasite into the next host during infection. To investigate this hypothesis, we used a proteomic approach to analyze excretion/secretion products (ESP) from Fasciola hepatica newly excysted juveniles (NEJ) derived from two intermediate host species, Lymnaea viatrix and Pseudosuccinea columella. The two analyzed proteomes showed differences in identity, abundance, and functional classification of the proteins. This observation could be due to differences in the biological cycle of the parasite in the host, environmental aspects, and/or host-dependent factors. Categories such as protein modification machinery, protease inhibitors, signal transduction, and cysteine-rich proteins showed different abundance between samples. More specifically, differences in abundance of individual proteins such as peptidyl-prolyl cis-trans isomerase, thioredoxin, cathepsin B, cathepsin L, and Kunitz-type inhibitors were identified. Based on the differences identified between NEJ ESP samples, we can conclude that the intermediate host is a factor influencing the proteomic profile of ESP in F. hepatica.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Carbonic Anhydrases classification
Carbonic Anhydrases metabolism
Helminth Proteins classification
Larva metabolism
Peptide Hydrolases classification
Peptide Hydrolases metabolism
Peroxiredoxins classification
Peroxiredoxins metabolism
Protease Inhibitors classification
Protease Inhibitors metabolism
Receptors, Cell Surface classification
Receptors, Cell Surface metabolism
Fasciola hepatica metabolism
Helminth Proteins metabolism
Lymnaea parasitology
Proteomics
Snails parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 201
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 31022392
- Full Text :
- https://doi.org/10.1016/j.exppara.2019.04.004