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Transcriptional profiling of innate immune responses in sheep PBMCs induced by Haemonchus contortus soluble extracts.
- Source :
-
Parasites & vectors [Parasit Vectors] 2019 Apr 25; Vol. 12 (1), pp. 182. Date of Electronic Publication: 2019 Apr 25. - Publication Year :
- 2019
-
Abstract
- Background: Pattern recognition receptors (PRRs) can recognize pathogen-associated molecular patterns and activate downstream signalling pathways, resulting in modulation of host immunity against pathogens. Here, we investigated whether PRR-mediated recognition is involved in host immune responses to the blood-feeding nematode Haemonchus contortus.<br />Methods: During blood-feeding, H. contortus secretes immune-modulating antigens into host blood. Therefore, we stimulated sheep peripheral blood mononuclear cells (PBMCs) with H. contortus soluble extract (HcAg) and performed transcriptional profiling.<br />Results: HcAg upregulated two genetically linked CLRs (CLEC2L and KLRG2), two NLRs attenuating inflammation (NLRP12 and NLRC3) and one G protein-coupled receptor with potent anti-inflammatory effects (HCAR2). Furthermore, several Th2-related transcription factors (ATF3, IRF4, BCL3 and NFATC) were also upregulated, which may confer anti-inflammatory type 2 immune responses to HcAg.<br />Conclusions: Together, our preliminary studies provide new insights into how the host innate immune system controls type 2 immunity to H. contortus. Further work will be needed to identify H. contortus products recognized by the host innate immune system and determine the Th2 polarization ability of these putative PRR ligands.
- Subjects :
- Animals
Antigens, Helminth immunology
Gene Expression Profiling
Haemonchiasis blood
Helminth Proteins immunology
Host-Pathogen Interactions
Leukocytes, Mononuclear parasitology
NLR Proteins genetics
Receptors, Pattern Recognition genetics
Receptors, Pattern Recognition immunology
Sheep
Sheep Diseases immunology
Sheep Diseases parasitology
Transcription Factors genetics
Haemonchiasis veterinary
Haemonchus chemistry
Helminth Proteins pharmacology
Immunity, Innate
Leukocytes, Mononuclear immunology
Tissue Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1756-3305
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Parasites & vectors
- Publication Type :
- Academic Journal
- Accession number :
- 31023355
- Full Text :
- https://doi.org/10.1186/s13071-019-3441-8