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Resistance risk assessment for fludioxonil in Sclerotinia homoeocarpa in China.
- Source :
-
Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2019 May; Vol. 156, pp. 123-128. Date of Electronic Publication: 2019 Feb 12. - Publication Year :
- 2019
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Abstract
- Sclerotinia homoeocarpa causes dollar spot disease on turfgrass and is a serious problem on many species worldwide. Fludioxonil, a phenylpyrrole fungicide, is not currently registered for dollar spot control in China. In this study, the baseline sensitivity to fludioxonil was established using an in vitro assay for 105 isolates of S. homoeocarpa collected from 10 locations in different regions of China. Results indicate that the frequency distribution of effective concentration for 50% inhibition of mycelial growth (EC <subscript>50</subscript> ) values of the S. homoeocarpa isolates was unimodal (W = 0.9847, P = .2730). The mean EC <subscript>50</subscript> value was 0.0020 ± 0.0006 μg/ml with a range from 0.0003 to 0.0035 μg/ml. A total of 7 fludioxonil-resistant mutants were obtained in laboratory, the mutants were stable in fludioxonil sensitivity after the 10th transfer, with resistance factor (RF) ranging from 4.320 to >13,901.4. The mutants showed a positive cross-resistance between fludioxonil and the dicarboximide fungicide iprodione, but not propiconazole, fluazinam, and thiophanate-methyl. When mycelial growth rate, pathogenicity and osmotic sensitivity were assessed, the mutants decreased in the fitness compared with their parental isolates. Sequence alignment of the histidine kinase gene Shos1 revealed a 13-bp fragment deletion only in one mutant, no mutations were observed on Shos1 in the rest resistant mutants.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Aminoimidazole Carboxamide analogs & derivatives
Aminoimidazole Carboxamide pharmacology
Aminopyridines pharmacology
Ascomycota genetics
China
Drug Resistance, Fungal genetics
Fungal Proteins genetics
Gene Expression Regulation, Fungal drug effects
Gene Expression Regulation, Fungal genetics
Hydantoins pharmacology
Mutation genetics
Thiophanate pharmacology
Triazoles pharmacology
Ascomycota drug effects
Ascomycota metabolism
Dioxoles pharmacology
Fungicides, Industrial pharmacology
Pyrroles pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9939
- Volume :
- 156
- Database :
- MEDLINE
- Journal :
- Pesticide biochemistry and physiology
- Publication Type :
- Academic Journal
- Accession number :
- 31027571
- Full Text :
- https://doi.org/10.1016/j.pestbp.2019.02.011