Back to Search
Start Over
Morphophysiological responses of detached and adhered biofilms of Pseudomonas fluorescens to acidic electrolyzed water.
- Source :
-
Food microbiology [Food Microbiol] 2019 Sep; Vol. 82, pp. 89-98. Date of Electronic Publication: 2019 Jan 17. - Publication Year :
- 2019
-
Abstract
- Pseudomonas spp. have emerged as the main spoilage bacteria, with many strains easily forming biofilms on food-contact surfaces and causing cross-contamination. The efficacy of disinfectants against bacteria is usually tested with planktonic cells; however, the disinfection tolerance of biofilms, especially detached biofilms, remains unknown. Here, we investigated the tolerance responses of detached and adhered biofilms of Pseudomonas fluorescens to acidic electrolyzed water (AEW) by determining tolerance responses by plate counting, comparing them using a Weibull model, and verifying changes in bacterial morphology by scanning electron microscopy. The experimental data and the responses calculated using Weibull a (scale) and b (shape) parameters agreed well (R <superscript>2</superscript> values: 0.974-0.999), and we found that AEW exhibited effective antimicrobial activity against P. fluorescens, with adhered biofilms were more resistant than detached biofilms and planktonic cells. Additionally, AEW increased the bacterial membrane permeability and decreased the membrane potential, intracellular ATP concentrations, and intracellular pH while also triggering the disruption of extracellular polymeric substances. These results demonstrated that the morphophysiological responses of detached and adhered biofilms differed significantly and provided information on disinfectant-resistance strategies potentially beneficial to the development of novel disinfection approaches.<br /> (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Subjects :
- Acids chemistry
Cell Membrane Permeability
Colony Count, Microbial
Disinfection methods
Membrane Potentials drug effects
Microscopy, Electron, Scanning
Pseudomonas fluorescens physiology
Water chemistry
Biofilms drug effects
Disinfectants pharmacology
Electrolysis
Pseudomonas fluorescens drug effects
Water pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9998
- Volume :
- 82
- Database :
- MEDLINE
- Journal :
- Food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31027824
- Full Text :
- https://doi.org/10.1016/j.fm.2019.01.007