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Molecular markers based on sequence variation in BoFLC1.C9 for characterizing early- and late-flowering cabbage genotypes.
- Source :
-
BMC genetics [BMC Genet] 2019 Apr 27; Vol. 20 (1), pp. 42. Date of Electronic Publication: 2019 Apr 27. - Publication Year :
- 2019
-
Abstract
- Background: Cabbage (Brassica oleracea var. capitata) is popular worldwide for consumption as a leafy vegetable. Premature flowering is triggered by low temperature, and deteriorates quality of cabbage as vegetable. In general, growers prefer late-flowering varieties to assure good quality compact head. Here, we report BoFLC1.C9 as a gene with clear sequence variation between cabbage lines with different flowering times, and proposed as molecular marker to characterize early- and late-flowering cabbage lines.<br />Results: We identified sequence variation of 67 bp insertions in intron 2, which were contributed in flowering time variation between two inbred lines through rapid down-regulation of the BoFLC1.C9 gene in early-flowering line compared to late-flowering one upon vernalization. One set of primer 'F7R7' proposed as marker, of which was explained with 83 and 80% of flowering time variation in 141 F <subscript>2</subscript> individuals and 20 commercial lines, respectively.<br />Conclusions: This F7R7 marker could be used as genetic tools to characterize flowering time variation and to select as well to develop early- and late-flowering cabbage cultivars.
Details
- Language :
- English
- ISSN :
- 1471-2156
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC genetics
- Publication Type :
- Academic Journal
- Accession number :
- 31029104
- Full Text :
- https://doi.org/10.1186/s12863-019-0740-1