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Arabidopsis ubiquitin-specific proteases UBP12 and UBP13 shape ORE1 levels during leaf senescence induced by nitrogen deficiency.
- Source :
-
The New phytologist [New Phytol] 2019 Aug; Vol. 223 (3), pp. 1447-1460. Date of Electronic Publication: 2019 Jun 17. - Publication Year :
- 2019
-
Abstract
- Nitrogen deficiency (-N) in plants triggers leaf senescence which is regulated by the transcription factor ORE1. Little is known about post-translational regulation of ORE1 in this process. Here, we show that UBP12/UBP13 (ubiquitin-specific protease 12/13) antagonize the action of NLA (nitrogen limitation adaptation) E3 ligase to maintain ORE1 homeostasis. In vitro pull-down and in vivo co-immunoprecipitation assays demonstrated specific binding between UBP12/UBP13 and ORE1. We further analyzed in various genotypes total Chl content and expression levels of senescence-related genes under -N conditions. We found that UBP12/UBP13 can deubiquitinate polyubiquitinated ORE1 in vitro and increase the stability of ORE1 in vivo in MG132/cycloheximide-chase experiments. Plants overexpressing UBP12/UBP13 display accelerated leaf senescence which is reversed by the ore1 mutation. By contrast, the senescence phenotype of plants overexpressing ORE1 is exacerbated by UBP12/UBP13 overexpression. The expression of senescence-related genes tracks the senescence phenotype. ORE1 protein levels can be elevated by UBP12/UBP13 overexpression but decreased in ubp12-2w/13-3. In conclusion, UBP12/UBP13 deubiquitinate ORE1 to stabilize this transcription factor and promote its activity as a positive regulator for leaf senescence under -N conditions. Our study shows that UBP12/UBP13 counteracts the effect of NLA E3 ligase to accelerate leaf senescence under nitrogen starvation.<br /> (© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.)
- Subjects :
- Arabidopsis genetics
Genotype
Models, Biological
Mutation genetics
Phenotype
Polyubiquitin metabolism
Protein Binding
Protein Stability
Ubiquitination
Arabidopsis enzymology
Arabidopsis Proteins metabolism
Endopeptidases metabolism
Nitrogen deficiency
Plant Leaves enzymology
Plant Leaves growth & development
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 223
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 31050353
- Full Text :
- https://doi.org/10.1111/nph.15879