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Sulforaphane prevents PC12 cells from oxidative damage via the Nrf2 pathway.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2019 Jun; Vol. 19 (6), pp. 4890-4896. Date of Electronic Publication: 2019 Apr 10. - Publication Year :
- 2019
-
Abstract
- The aim of this study was to investigate the protective effect of sulforaphane (SFN) on 1‑methyl‑4‑phenyl pyridine ion (MPP+)‑induced cytotoxicity and to investigate its possible mechanisms.<br />Methods: PC12 cell toxicity induced by MPP+ served as a cell model of Parkinson's diseases. The cell culture + experiments were divided into four groups based on the different treatments, namely, vehicle control, SFN, MPP+ and SFN pretreatment plus MPP+. Cell viability and apoptosis were examined by MTT assay and flow cytometry, respectively. Expressions of nuclear factor erythroid 2‑related factor 2 (Nrf2), heme oxygenase 1 (HO‑1) and nicotinamide quinone oxidoreductase 1 (NQO1) were detected using western blotting.<br />Results: MPP+ reduced the survival rate of PC12 cells in a dose‑ and time‑dependent manner. After 24‑h treatment with 500 µmol/l MPP+, the survival rate of PC12 cells decreased to 58.2±0.03% of that in the control groups. Under the same conditions MPP+ resulted in significant apoptosis of PC12 cells (apoptosis rate: 30.4±0.6%). However, SFN pretreatment significantly attenuated the cell damage induced by MPP+. Furthermore, it was demonstrated that SFN reversed the reduction of Nrf2, HO‑1 and NQO1 expression induced by MPP+.<br />Conclusion: SFN may protect PC12 cells from MPP+‑induced damage via activating the Nrf2‑ARE (antioxidant responsive element) pathway.
- Subjects :
- 1-Methyl-4-phenylpyridinium toxicity
Animals
Antioxidant Response Elements
Antioxidants pharmacology
Antiparkinson Agents pharmacology
Apoptosis drug effects
Cell Survival drug effects
Heme Oxygenase-1 metabolism
Isothiocyanates administration & dosage
NAD(P)H Dehydrogenase (Quinone) metabolism
Parkinson Disease drug therapy
Rats
Reactive Oxygen Species metabolism
Signal Transduction drug effects
Sulfoxides
Survival Rate
Time Factors
Isothiocyanates pharmacology
NF-E2-Related Factor 2 metabolism
Oxidative Stress drug effects
PC12 Cells drug effects
Protective Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 31059012
- Full Text :
- https://doi.org/10.3892/mmr.2019.10148