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Development of a new monoclonal antibody specific to mouse Vγ6 chain.
- Source :
-
Life science alliance [Life Sci Alliance] 2019 May 07; Vol. 2 (3). Date of Electronic Publication: 2019 May 07 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene-transfected T-cell line lacking TCR as well as of Vγ1 <superscript>-</superscript> Vγ4 <superscript>-</superscript> Vγ5 <superscript>-</superscript> Vγ7 <superscript>-</superscript> γδ T cells and the CD3 <superscript>high</superscript> TCRδ <superscript>int</superscript> γδ T cells in various organs. The location of Vγ6 <superscript>+</superscript> γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice.<br /> (© 2019 Hatano et al.)
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Antibody Formation immunology
Cell Line, Tumor
Female
Immunization
Immunophenotyping
Mice
Receptors, Antigen, T-Cell, gamma-delta immunology
Receptors, Antigen, T-Cell, gamma-delta metabolism
T-Lymphocytes immunology
T-Lymphocytes metabolism
Antibodies, Monoclonal pharmacology
Antibody Specificity immunology
Receptors, Antigen, T-Cell, gamma-delta antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 2575-1077
- Volume :
- 2
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Life science alliance
- Publication Type :
- Academic Journal
- Accession number :
- 31064767
- Full Text :
- https://doi.org/10.26508/lsa.201900363