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Physical and functional cell-matrix uncoupling in a developing tissue under tension.
- Source :
-
Development (Cambridge, England) [Development] 2019 Jun 03; Vol. 146 (11). Date of Electronic Publication: 2019 Jun 03. - Publication Year :
- 2019
-
Abstract
- Tissue mechanics play a crucial role in organ development. They rely on the properties of cells and the extracellular matrix (ECM), but the relative physical contribution of cells and ECM to morphogenesis is poorly understood. Here, we have analyzed the behavior of the peripodial epithelium (PE) of the Drosophila leg disc in the light of the dynamics of its cellular and ECM components. The PE undergoes successive changes during leg development, including elongation, opening and removal to free the leg. During elongation, we found that the ECM and cell layer are progressively uncoupled. Concomitantly, the tension, mainly borne by the ECM at first, builds up in the cell monolayer. Then, each layer of the peripodial epithelium is removed by an independent mechanism: while the ECM layer withdraws following local proteolysis, cellular monolayer withdrawal is independent of ECM degradation and is driven by myosin II-dependent contraction. These results reveal a surprising physical and functional cell-matrix uncoupling in a monolayer epithelium under tension during development.This article has an associated 'The people behind the papers' interview.<br />Competing Interests: Competing interestsThe authors declare no competing or financial interests.<br /> (© 2019. Published by The Company of Biologists Ltd.)
- Subjects :
- Animals
Animals, Genetically Modified
Basement Membrane embryology
Basement Membrane growth & development
Biomechanical Phenomena
Body Patterning physiology
Cell Communication physiology
Cell Proliferation
Drosophila melanogaster growth & development
Embryo, Nonmammalian
Hindlimb growth & development
Myosin Type II physiology
Proteolysis
Surface Tension
Drosophila melanogaster embryology
Epithelium embryology
Epithelium growth & development
Extracellular Matrix physiology
Hindlimb embryology
Morphogenesis physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 146
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 31064785
- Full Text :
- https://doi.org/10.1242/dev.172577