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[Study on DNA methylation profiles in non-syndromic cleft lip/palate based on bioinformatics].
- Source :
-
Shanghai kou qiang yi xue = Shanghai journal of stomatology [Shanghai Kou Qiang Yi Xue] 2019 Feb; Vol. 28 (1), pp. 57-62. - Publication Year :
- 2019
-
Abstract
- Purpose: To study DNA methylation patterns of non-syndromic cleft lip/palate(NSCL/P) using bioinformatic methods, including methylated positions and regions.<br />Methods: Whole blood DNA methylation data of NSCL/P samples was download from Gene Expression Omnibus(GEO) database, including 67 NSCL/P cases and 59 controls without birth defects. Data analysis included ①data cleaning, such as probes filtering, quality control and normalization; ②differential methylation analysis, including methylated positions and regions; ③Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis on differential methylated genes. R 3.4.3 software was used for data cleaning, differential methylated positions and regions analysis. DAVID6.8 tool was used for GO and KEGG analysis.<br />Results: 814 differential methylated positions were detected (adjusted P<0.001,|Δβ|>0.125), of which 178 were hypermethylated in NSCL/P patients, and 636 were hypomethylated. In addition, 386 differential methylated regions were identified (P<0.05), of which 204 were hypermethylation regions and 182 were hypomethylation regions. GO analysis showed that 38 differential methylated genes were involved in 7 kinds of biological processes, 163 differential methylated genes were involved in 3 kinds of molecular functions, and 114 differential methylated genes were involved in 3 kinds of cellular components (P<0.01). KEGG analysis showed that 59 differential methylated genes were involved in 9 kinds of signal pathways.<br />Conclusions: Abnormal DNA methylation patterns of NSCL/P might be an important epigenetic mechanism affecting the development of NSCL/P. This study might contribute to the identification of identification of biomarkers and targeted interventions of NSCL/P.
Details
- Language :
- Chinese
- ISSN :
- 1006-7248
- Volume :
- 28
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Shanghai kou qiang yi xue = Shanghai journal of stomatology
- Publication Type :
- Academic Journal
- Accession number :
- 31081001