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Recent Advances in Understanding σ70-Dependent Transcription Initiation Mechanisms.
- Source :
-
Journal of molecular biology [J Mol Biol] 2019 Sep 20; Vol. 431 (20), pp. 3947-3959. Date of Electronic Publication: 2019 May 11. - Publication Year :
- 2019
-
Abstract
- Prokaryotic transcription is one of the most studied biological systems, with relevance to many fields including the development and use of antibiotics, the construction of synthetic gene networks, and the development of many cutting-edge methodologies. Here, we discuss recent structural, biochemical, and single-molecule biophysical studies targeting the mechanisms of transcription initiation in bacteria, including the formation of the open complex, the reaction of initial transcription, and the promoter escape step that leads to elongation. We specifically focus on the mechanisms employed by the RNA polymerase holoenzyme with the housekeeping sigma factor σ <superscript>70</superscript> . The recent progress provides answers to long-held questions, identifies intriguing new behaviours, and opens up fresh questions for the field of transcription.<br /> (Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- DNA, Bacterial genetics
DNA, Bacterial metabolism
DNA-Directed RNA Polymerases chemistry
DNA-Directed RNA Polymerases genetics
Multienzyme Complexes chemistry
Multienzyme Complexes genetics
Multienzyme Complexes metabolism
Promoter Regions, Genetic
Sigma Factor chemistry
Sigma Factor genetics
Bacteria enzymology
Bacteria metabolism
DNA-Directed RNA Polymerases metabolism
Sigma Factor metabolism
Transcription Initiation, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8638
- Volume :
- 431
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Journal of molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 31082441
- Full Text :
- https://doi.org/10.1016/j.jmb.2019.04.046