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Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.
- Source :
-
Anesthesia and analgesia [Anesth Analg] 2019 Nov; Vol. 129 (5), pp. e150-e154. - Publication Year :
- 2019
-
Abstract
- Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.
- Subjects :
- Cerebrospinal Fluid cytology
GPI-Linked Proteins analysis
Humans
Lipopolysaccharide Receptors analysis
Pilot Projects
Postoperative Cognitive Complications etiology
Receptors, IgG analysis
Flow Cytometry methods
Monocytes immunology
Postoperative Cognitive Complications cerebrospinal fluid
Subjects
Details
- Language :
- English
- ISSN :
- 1526-7598
- Volume :
- 129
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Anesthesia and analgesia
- Publication Type :
- Academic Journal
- Accession number :
- 31085945
- Full Text :
- https://doi.org/10.1213/ANE.0000000000004179