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Analysis of an improved Cyanophora paradoxa genome assembly.
Analysis of an improved Cyanophora paradoxa genome assembly.
- Source :
-
DNA research : an international journal for rapid publication of reports on genes and genomes [DNA Res] 2019 Aug 01; Vol. 26 (4), pp. 287-299. - Publication Year :
- 2019
-
Abstract
- Glaucophyta are members of the Archaeplastida, the founding group of photosynthetic eukaryotes that also includes red algae (Rhodophyta), green algae, and plants (Viridiplantae). Here we present a high-quality assembly, built using long-read sequences, of the ca. 100 Mb nuclear genome of the model glaucophyte Cyanophora paradoxa. We also conducted a quick-freeze deep-etch electron microscopy (QFDEEM) analysis of C. paradoxa cells to investigate glaucophyte morphology in comparison to other organisms. Using the genome data, we generated a resolved 115-taxon eukaryotic tree of life that includes a well-supported, monophyletic Archaeplastida. Analysis of muroplast peptidoglycan (PG) ultrastructure using QFDEEM shows that PG is most dense at the cleavage-furrow. Analysis of the chlamydial contribution to glaucophytes and other Archaeplastida shows that these foreign sequences likely played a key role in anaerobic glycolysis in primordial algae to alleviate ATP starvation under night-time hypoxia. The robust genome assembly of C. paradoxa significantly advances knowledge about this model species and provides a reference for exploring the panoply of traits associated with the anciently diverged glaucophyte lineage.<br /> (© The Author(s) 2019. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.)
Details
- Language :
- English
- ISSN :
- 1756-1663
- Volume :
- 26
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- DNA research : an international journal for rapid publication of reports on genes and genomes
- Publication Type :
- Academic Journal
- Accession number :
- 31098614
- Full Text :
- https://doi.org/10.1093/dnares/dsz009