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Analysis of an improved Cyanophora paradoxa genome assembly.

Analysis of an improved Cyanophora paradoxa genome assembly.

Authors :
Price DC
Goodenough UW
Roth R
Lee JH
Kariyawasam T
Mutwil M
Ferrari C
Facchinelli F
Ball SG
Cenci U
Chan CX
Wagner NE
Yoon HS
Weber APM
Bhattacharya D
Source :
DNA research : an international journal for rapid publication of reports on genes and genomes [DNA Res] 2019 Aug 01; Vol. 26 (4), pp. 287-299.
Publication Year :
2019

Abstract

Glaucophyta are members of the Archaeplastida, the founding group of photosynthetic eukaryotes that also includes red algae (Rhodophyta), green algae, and plants (Viridiplantae). Here we present a high-quality assembly, built using long-read sequences, of the ca. 100 Mb nuclear genome of the model glaucophyte Cyanophora paradoxa. We also conducted a quick-freeze deep-etch electron microscopy (QFDEEM) analysis of C. paradoxa cells to investigate glaucophyte morphology in comparison to other organisms. Using the genome data, we generated a resolved 115-taxon eukaryotic tree of life that includes a well-supported, monophyletic Archaeplastida. Analysis of muroplast peptidoglycan (PG) ultrastructure using QFDEEM shows that PG is most dense at the cleavage-furrow. Analysis of the chlamydial contribution to glaucophytes and other Archaeplastida shows that these foreign sequences likely played a key role in anaerobic glycolysis in primordial algae to alleviate ATP starvation under night-time hypoxia. The robust genome assembly of C. paradoxa significantly advances knowledge about this model species and provides a reference for exploring the panoply of traits associated with the anciently diverged glaucophyte lineage.<br /> (© The Author(s) 2019. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.)

Details

Language :
English
ISSN :
1756-1663
Volume :
26
Issue :
4
Database :
MEDLINE
Journal :
DNA research : an international journal for rapid publication of reports on genes and genomes
Publication Type :
Academic Journal
Accession number :
31098614
Full Text :
https://doi.org/10.1093/dnares/dsz009