Colorimetric evaluation of the hydroxyl radical scavenging ability of antioxidants using carbon-confined CoO x as a highly active peroxidase mimic.

The authors present a colorimetric method for the evaluation of the hydroxyl radical scavenging capability of antioxidants by exploiting carbon-confined mixed cobalt oxide nanoparticles (denoted as C-confined CoO _x NPs) as a novel peroxidase mimic. The nanozyme can be prepared from the metal-organic framework ZIF-67 by calcination at a moderate temperature. It exhibits peroxidase-mimicking activity and catalyzes the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to form a blue product in the presence of H ₂ O ₂ via generation of hydroxyl radicals. However, in the presence of an antioxidant, the color reaction is suppressed due to scavenging of hydroxyl radicals by the antioxidant. Based on this principle, the hydroxy radical scavenging ability of glutathione (GSH), cysteine (Cys), tannic acid (TA) and tea polyphenols (TP) was assessed. It was found that these antioxidants can scavenge hydroxyl radicals in the following decreasing order: TA>Cys>GSH>TP. The assay was also used to quantify the antioxidative power of common fruit extracts. Graphical abstract Schematic presentation for evaluating the hydroxyl radical scavenging ability of different antioxidants using carbon-confined mixed cobalt oxide nanoparticles (denoted as C-confined CoO _x NPs) as a highly active peroxidase mimic. With excellent activity, the C-confined CoO _x NPs together with the visible peroxidase reaction can be employed as a powerful tool to rapidly screen appropriate antioxidants from natural samples and measure their activity for guiding their usage in related products.