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The Long Noncoding RNA Pnky Is a Trans-acting Regulator of Cortical Development In Vivo.
- Source :
-
Developmental cell [Dev Cell] 2019 May 20; Vol. 49 (4), pp. 632-642.e7. - Publication Year :
- 2019
-
Abstract
- While it is now appreciated that certain long noncoding RNAs (lncRNAs) have important functions in cell biology, relatively few have been shown to regulate development in vivo, particularly with genetic strategies that establish cis versus trans mechanisms. Pnky is a nuclear-enriched lncRNA that is transcribed divergently from the neighboring proneural transcription factor Pou3f2. Here, we show that conditional deletion of Pnky from the developing cortex regulates the production of projection neurons from neural stem cells (NSCs) in a cell-autonomous manner, altering postnatal cortical lamination. Surprisingly, Pou3f2 expression is not disrupted by deletion of the entire Pnky gene. Moreover, expression of Pnky from a BAC transgene rescues the differential gene expression and increased neurogenesis of Pnky-knockout NSCs, as well as the developmental phenotypes of Pnky-deletion in vivo. Thus, despite being transcribed divergently from a key developmental transcription factor, the lncRNA Pnky regulates development in trans.<br /> (Published by Elsevier Inc.)
- Subjects :
- Animals
Brain metabolism
Cerebral Cortex metabolism
Female
Interneurons metabolism
Male
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins genetics
Neurogenesis genetics
Neurons metabolism
POU Domain Factors genetics
RNA, Long Noncoding metabolism
Trans-Activators genetics
Trans-Activators metabolism
Transcription Factors metabolism
Cerebral Cortex embryology
Neural Stem Cells metabolism
RNA, Long Noncoding genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1551
- Volume :
- 49
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Developmental cell
- Publication Type :
- Academic Journal
- Accession number :
- 31112699
- Full Text :
- https://doi.org/10.1016/j.devcel.2019.04.032