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M10 peptide attenuates silica-induced pulmonary fibrosis by inhibiting Smad2 phosphorylation.
- Source :
-
Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2019 Aug 01; Vol. 376, pp. 46-57. Date of Electronic Publication: 2019 May 21. - Publication Year :
- 2019
-
Abstract
- Silica-induced pulmonary fibrosis is a kind of worldwide occupational disease, and there is no effective treatment at present. Peptide therapy has attracted significant attention due to its simple structure, high selectiveness, strong bioactivity, relative safety, and high patient tolerance. In this study, we first confirmed that M10, a 10 amino acid peptide, has anti-fibrotic effects during the early and late stages of silica-induced fibrosis in mouse models and then partly explored the underlying mechanisms in vitro. M10 was detected in both the cell cytoplasm and nuclei. M10 showed no cytotoxicity to pulmonary epithelial cells and fibroblasts at the given concentrations. Functionally, M10 can reverse the silica-induced EMT process in epithelial cells and decrease TGF-β1-stimulated fibroblast activation. Further mechanism investigations supported that M10 can block TGF-β1 signalling by inhibiting phosphorylation of Smad2 protein in vitro and in vivo. All of the results indicate that M10 peptide may be a new method for the treatment of silica-induced pulmonary fibrosis.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line
Disease Models, Animal
Epithelial Cells
Epithelial-Mesenchymal Transition drug effects
Fibroblasts drug effects
Fibroblasts physiology
Male
Mice
Mice, Inbred C57BL
NIH 3T3 Cells
Peptides therapeutic use
Phosphorylation drug effects
Pulmonary Fibrosis chemically induced
Signal Transduction drug effects
Transforming Growth Factor beta1 metabolism
Transforming Growth Factor beta1 pharmacology
Peptides pharmacology
Pulmonary Fibrosis drug therapy
Silicon Dioxide toxicity
Smad2 Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0333
- Volume :
- 376
- Database :
- MEDLINE
- Journal :
- Toxicology and applied pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 31125577
- Full Text :
- https://doi.org/10.1016/j.taap.2019.05.015