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Molecular characterization of Peste des Petits ruminants virus isolated from four outbreaks occurred in southern Iran.
- Source :
-
BMC veterinary research [BMC Vet Res] 2019 May 28; Vol. 15 (1), pp. 177. Date of Electronic Publication: 2019 May 28. - Publication Year :
- 2019
-
Abstract
- Background: Peste des petits ruminants (PPR) is a severe infectious disease in both domestic and wild small ruminants. Due to its heavy economic burden and hence social and health impacts on human populations, Food and Agriculture Organization of the United Nations (FAO) and The World Organization for Animal Health (OIE) have targeted PPR for eradication by 2030. In order to plan and implement a successful eradication program, factual status assessments prior to devising disease control strategies is a vital criterion. The aim of this study was to isolate and characterize PPR virus from a rising wave of outbreaks in southern Iran.<br />Results: Twenty-one clinical samples, including blood as well as oral, nasal and ocular swabs were collected from ten sick animals in 4 various herds and were examined with ELISA and RT-PCR for the presence of PPR virus antigen and genome, respectively. The virus was successfully isolated in primary lamb kidney cell culture and identified by RT-PCR. Phylogenetic analysis of the sequenced N genes revealed that, while the earliest reports of Iran's outbreaks were grouped into clusters with Saudi Arabia, Turkey and Africa, in this study reported sequences were grouped with samples from Pakistan, Tajikistan and China in particular. This observation suggests a shift in PPRV flow from the western borders of the country to the eastern neighboring countries.<br />Conclusions: Lineage IV of PPR virus is presently circulating in Iran, with certain levels of genetic diversity. Present study along with previous reports demonstrates the dispersal patterns and movements of PPR virus, which highlights the reversal pattern of virus flow in recent years. Such information is necessary to understand PPRV molecular epidemiology and to develop more proper control strategies to eradicate the disease in the planned time.
- Subjects :
- Animals
Antigens, Viral analysis
Cells, Cultured
Enzyme-Linked Immunosorbent Assay veterinary
Genome, Viral
Goat Diseases epidemiology
Goat Diseases virology
Goats
Iran epidemiology
Peste-des-Petits-Ruminants virology
Peste-des-petits-ruminants virus isolation & purification
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction veterinary
Sheep
Sheep Diseases epidemiology
Disease Outbreaks veterinary
Peste-des-Petits-Ruminants epidemiology
Peste-des-petits-ruminants virus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1746-6148
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 31138202
- Full Text :
- https://doi.org/10.1186/s12917-019-1920-y